UCP3 Antibody - #DF6443

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製品: UCP3 Antibody
カタログ: DF6443
タンパク質の説明: Rabbit polyclonal antibody to UCP3
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Horse, Dog
分子量: 34kDa; 34kD(Calculated).
ユニプロット: P55916
RRID: AB_2838406

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Horse(88%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
UCP3 Antibody detects endogenous levels of total UCP3.
RRID:
AB_2838406
引用形式: Affinity Biosciences Cat# DF6443, RRID:AB_2838406.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Mitochondrial uncoupling protein 3; SLC25A9; Solute carrier family 25 member 9; UCP 3; UCP3; UCP3_HUMAN; Uncoupling protein 3; Uncoupling protein 3 mitochondrial proton carrier;

免疫原

免疫原:

A synthesized peptide derived from human UCP3, corresponding to a region within C-terminal amino acids.

Uniprot:

P55916(UCP3_HUMAN) >>Visit HPA database.

遺伝子(ID):
UCP3(7352)
発現特異性:
P55916 UCP3_HUMAN:

Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.

タンパク質の説明:
The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.
タンパク質配列:
MVGLKPSDVPPTMAVKFLGAGTAACFADLVTFPLDTAKVRLQIQGENQAVQTARLVQYRGVLGTILTMVRTEGPCSPYNGLVAGLQRQMSFASIRIGLYDSVKQVYTPKGADNSSLTTRILAGCTTGAMAVTCAQPTDVVKVRFQASIHLGPSRSDRKYSGTMDAYRTIAREEGVRGLWKGTLPNIMRNAIVNCAEVVTYDILKEKLLDYHLLTDNFPCHFVSAFGAGFCATVVASPVDVVKTRYMNSPPGQYFSPLDCMIKMVAQEGPTAFYKGFTPSFLRLGSWNVVMFVTYEQLKRALMKVQMLRESPF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Dog
88
Bovine
75
Sheep
75
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.

細胞の位置付け:

Mitochondrion inner membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.

タンパク質ファミリー:

Belongs to the mitochondrial carrier (TC 2.A.29) family.

参考文献

1). Berberine attenuates the abnormal ectopic lipid deposition in skeletal muscle. Free radical biology & medicine, 2020 (PubMed: 32745766) [IF=7.1]

Application: WB    Species: mouse    Sample: skeletal muscle

Fig. 3. |Berberine promotes the expression of PGC-1α and other factors related to mitochondrial biogenesis and function in skeletal muscle.E: Representative western blots showing the key protein changes in the skeletal muscle of db/db mice treated with berberine or vehicle. All data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus db/db control mice.

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