AIF1/IBA1 Antibody - #DF6442

Figure 2 BDD exerts antidepressant effects by inhibiting the prefrontal Th17 cell-driven RORγt /Act1/TRAF6 pathway mediates microglial activation. A) Sucrose preference (%) in the SPT. B) Immobility time in the FST. C) Retention time in the open field center. D) Percentage of retention time in the open arm. E) Levels of IL-1β, IL-17A, and IL-10 in the mPFC of control or CUMS mice after treatment with saline or BDD. F) Representative flow cytometric analysis and statistical result of CD4+ Th17 cells contents within the mPFC. G) Fluorescence micrographs of DAPI (blue) and RORγt in the mPFC (×40 magnification, scale bar=10 µm). Quantitative analysis of RORγt, Iba1, iNOS, Arg-1, IL-17AR, Act1, and TRAF6 mRNAs H) level and protein expression of RORγt, Iba1, IL-17AR, Act1, and TRAF6 I) in the mPFC of each group of mice. GAPDH was set as the internal control. Data for individual animals are displayed as mean ± SEM (n=6 per/group). J) Immunofluorescence double staining of retinal sections with Iba1 (blue), IL-17AR (red), Act1 (red) or TRAF6 (red) and the ratio of IL-17AR+ Iba1+ / Iba1+ cells, Act1+ Iba1+ / Iba1+ cells and TRAF6+ Iba1+ / Iba1+ cells. Three non-overlapping fields of view were selected randomly in each slice under the fluorescence microscope. Arrows indicate the positive cells. Histogram is the quantification of the percentage of positive cells (n=3 per/group, 2 sections/animal). ** p

Fig. 5 Lateral ventricle administration of BMSC-exos inhibited the proliferation and activation of microglia in the hippocampus of mice injected with STZ. A Fluorescence detection of microglia marker IBA1 in hippocampus B Quantification of the number of IBA1 positive cells. C Microglia in different states (left: resting state; right: activated state); D The ratio of activated microglia to the total number of microglia. Data are presented as means ± SEM, with n = 3 in each group

Fig. 8 Effects of HRS treatment on protein expressions in septic rats 48 h after LPS challenge. A Representative images of protein expression levels by western blot analysis in each group. B–E Statistical representation of relative protein expressions of GFAP, IBA-1, BCL-2 and BAX; respectively. All data are presented as mean ± SD ****p < 0.0001, ***p < 0.001, **p < 0.005, *p < 0.05. HRS Hydrogen-rich saline, LPS Lipopolysaccharide, GFAP Glial fibrillary acidic protein, IBA-1 Ionised calcium binding adaptor molecule 1, BCL-2 B-cell lymphoma 2