OGG1 Antibody - #DF6401

(1)  
製品: OGG1 Antibody
カタログ: DF6401
タンパク質の説明: Rabbit polyclonal antibody to OGG1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat, Monkey
予測: Bovine, Sheep, Dog
分子量: 39kDa; 39kD(Calculated).
ユニプロット: O15527
RRID: AB_2838364

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Bovine(85%), Sheep(85%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
OGG1 Antibody detects endogenous levels of total OGG1.
RRID:
AB_2838364
引用形式: Affinity Biosciences Cat# DF6401, RRID:AB_2838364.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

8 hydroxyguanine DNA glycosylase; 8 oxoguanine DNA glycosylase 1; 8 oxoguanine DNA glycosylase; AP lyase; DNA (apurinic or apyrimidinic site) lyase; DNA apurinic or apyrimidinic site lyase; DNA lyase; DNA-(apurinic or apyrimidinic site) lyase; HMMH; HOGG 1; HOGG1; MMH; MUTM; N glycosylase; Ogg 1; Ogg1; OGG1_HUMAN; OGH 1; OGH1;

免疫原

免疫原:

A synthesized peptide derived from human OGG1, corresponding to a region within the internal amino acids.

Uniprot:

O15527(OGG1_HUMAN) >>Visit HPA database.

遺伝子(ID):
OGG1(4968)
発現特異性:
O15527 OGG1_HUMAN:

Ubiquitous.

タンパク質の説明:
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]
タンパク質配列:
MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
91
Bovine
85
Sheep
85
Horse
77
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15527 基板として

Site PTM Type Enzyme
S115 Phosphorylation
S183 Phosphorylation
K198 Ubiquitination
S231 Phosphorylation
S232 Phosphorylation
S280 Phosphorylation
K289 Ubiquitination
K297 Ubiquitination
S326 Phosphorylation
K338 Acetylation
K341 Acetylation

研究背景

機能:

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

細胞の位置付け:

Nucleus>Nucleoplasm. Nucleus speckle. Nucleus matrix.
Note: Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.

Nucleus.

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

タンパク質ファミリー:

Belongs to the type-1 OGG1 family.

研究領域

· Genetic Information Processing > Replication and repair > Base excision repair.

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