SDC1 Antibody - #DF6367

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製品: SDC1 Antibody
カタログ: DF6367
タンパク質の説明: Rabbit polyclonal antibody to SDC1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 33kDa; 32kD(Calculated).
ユニプロット: P18827
RRID: AB_2838331

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
SDC1 Antibody detects endogenous levels of total SDC1.
RRID:
AB_2838331
引用形式: Affinity Biosciences Cat# DF6367, RRID:AB_2838331.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD_antigen; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; SDC; Sdc1; SDC1_HUMAN; SYND1; Syndecan 1; syndecan proteoglycan 1; Syndecan-1;

免疫原

免疫原:
Uniprot:

P18827(SDC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
SDC1(6382)
タンパク質の説明:
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
タンパク質配列:
MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAGGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
83
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18827 基板として

Site PTM Type Enzyme
T61 O-Glycosylation
T72 O-Glycosylation
T76 O-Glycosylation
S77 O-Glycosylation
T81 O-Glycosylation
T122 O-Glycosylation
T164 O-Glycosylation
S165 O-Glycosylation
T166 O-Glycosylation
S171 O-Glycosylation
T177 O-Glycosylation
T180 O-Glycosylation
S186 O-Glycosylation
T188 O-Glycosylation
T240 O-Glycosylation
K280 Ubiquitination
K281 Ubiquitination
S285 Phosphorylation
Y286 Phosphorylation
S287 Phosphorylation
K292 Ubiquitination
Y299 Phosphorylation
K301 Ubiquitination
K304 Ubiquitination
Y309 Phosphorylation

研究背景

機能:

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

PTMs:

Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

細胞の位置付け:

Membrane>Single-pass type I membrane protein. Secreted. Secreted>Extracellular exosome.
Note: Shedding of the ectodomain produces a soluble form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain). Interacts with MDK (By similarity).

タンパク質ファミリー:

Belongs to the syndecan proteoglycan family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

参考文献

1). LncRNA H19 deficiency protects against the structural damage of glomerular endothelium in diabetic nephropathy via Akt/eNOS pathway. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (PubMed: 35867533) [IF=3.0]
2). Repression of T Cell-Mediated Alloimmunity by CX-5461 via the 1 p53-DUSP5 Pathway.

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