製品: BHMT Antibody
カタログ: DF6348
タンパク質の説明: Rabbit polyclonal antibody to BHMT
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Dog, Chicken
分子量: 45kDa; 45kD(Calculated).
ユニプロット: Q93088
RRID: AB_2838312

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(89%), Horse(89%), Dog(100%), Chicken(89%)
クローナリティ:
Polyclonal
特異性:
BHMT Antibody detects endogenous levels of total BHMT.
RRID:
AB_2838312
引用形式: Affinity Biosciences Cat# DF6348, RRID:AB_2838312.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Betaine homocysteine methyltransferase; Betaine homocysteine S methyltransferase 1; Betaine homocysteine S methyltransferase; Betaine--homocysteine S-methyltransferase 1; BHMT; BHMT1; BHMT1_HUMAN; EC 2.1.1.5; Epididymis secretory sperm binding protein Li 61p; HEL S 61p;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q93088 BHMT1_HUMAN:

Found exclusively in liver and kidney.

タンパク質の説明:
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
タンパク質配列:
MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVLEKISGQEVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKVFLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGASIIGVNCHFDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
89
Bovine
89
Chicken
89
Sheep
78
Rabbit
78
Xenopus
67
Zebrafish
44
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q93088 基板として

Site PTM Type Enzyme
T45 Phosphorylation
Y289 Phosphorylation
Y305 Phosphorylation
S330 Phosphorylation
K340 Methylation
Y363 Phosphorylation
S366 Phosphorylation
K369 Acetylation

研究背景

機能:

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found exclusively in liver and kidney.

サブユニット構造:

Homotetramer.

研究領域

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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