MMP1 Antibody - #DF6325

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製品: MMP1 Antibody
カタログ: DF6325
タンパク質の説明: Rabbit polyclonal antibody to MMP1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 54kDa; 54kD(Calculated).
ユニプロット: P03956
RRID: AB_2838289

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(89%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(89%)
クローナリティ:
Polyclonal
特異性:
MMP1 Antibody detects endogenous levels of total MMP1.
RRID:
AB_2838289
引用形式: Affinity Biosciences Cat# DF6325, RRID:AB_2838289.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

27 kDa interstitial collagenase; CLG; CLGN; collagenase, fibroblast; collagenase, interstitial; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix Metalloproteinase 1; Matrix metalloproteinase-1; MMP 1; MMP-1; MMP1; MMP1_HUMAN; OTTHUMP00000045866;

免疫原

免疫原:

A synthesized peptide derived from human MMP1, corresponding to a region within C-terminal amino acids.

Uniprot:

P03956(MMP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
MMP1(4312)
タンパク質の説明:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
タンパク質配列:
MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Bovine
90
Sheep
90
Pig
89
Dog
89
Chicken
60
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

PTMs:

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Tyrosine phosphorylated in platelets by PKDCC/VLK.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究領域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

参考文献

1). Downregulated cytotoxic CD8+ T-cell identifies with the NKG2A-soluble HLA-E axis as a predictive biomarker and potential therapeutic target in keloids. Cellular & molecular immunology, 2022 (PubMed: 35039632) [IF=21.8]
2). A non-retinol retinoic acid receptor-γ (RAR-γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A-induced skin damage. British journal of pharmacology, 2022 (PubMed: 35731978) [IF=6.8]
3). Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro. Bioengineered, 2022 (PubMed: 35094658) [IF=4.2]

Application: WB    Species: Human    Sample: SW1353 cells and chondrocytes

Figure 4. TB-II inhibited the production of cartilage degrading enzymes in IL-1β-treated SW1353 cells and chondrocytes. The mRNA expression of (a) MMP-1, (b) MMP-3 and (c) MMP-13 were detected by qRT-PCR. (d-g) The protein level of MMP-1, MMP-3, and MMP-13 were detected by Western blot. GAPDH was conducted as a loading control. One-way ANOVA, ##p < 0.01, compared with control cells; $p < 0.05, $$p < 0.01, ns: not-significant, compared with IL-1β-treated cells.
4). Deciphering the pharmacological mechanisms of Fraxini Cortex for ulcerative colitis treatment based on network pharmacology and in vivo studies. BMC Complementary Medicine and Therapies, 2023 (PubMed: 37161415) [IF=3.9]

Application: WB    Species: Mouse    Sample:

Fig. 11 FC regulated the expression of key target genes. The protein expression levels of (A-B) IL-1β, COX2, (C-D) IL-17, RORγt, and (E-F) MMP1, MMP3 and MMP9 were measured by western blot. Data are shown as the mean ± SD, #P 
5). Ubiquitin-specific protease 49 attenuates IL-1β-induced rat primary chondrocyte apoptosis by facilitating Axin deubiquitination and subsequent Wnt/β-catenin signaling cascade inhibition. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32737772) [IF=3.5]

Application: WB    Species: rat    Sample: chondrocytes

FIGURE 2 |e) Western blot analysis (right panel) and band intensity analysis (left panel) of the protein levels of Survivin, Mmp1, and Mmp13 in Control+Vehicle, Vector+IL-1β, and oeUSP49 +IL-1β rat chondro-cytes. ***P<0.001 versus the Vector or Control+Vehicle group; ## and ### indicate P values less than 0.01 and 0.001,respectively, versus the Vec-tor+IL-1β group
6). RUNX2 facilitates aggressiveness and chemoresistance of triple negative breast cancer cells via activating MMP1. Frontiers in oncology, 2022 (PubMed: 36483054) [IF=3.5]

Application: WB    Species: Mouse    Sample:

Figure 8 MMP1 and RUNX2 showed positively correlation in relative mRNA and protein expression. (A) qRT-PCR analysis of the expression of RUNX2 and MMP1 in various breast cancer cell lines. (B, C) qRT-PCR and western-blotting analyses of RUNX2 (B) and MMP1 (C) expression in MDA-MB-231-Re cells or MDA-MB-231-Pa cells. (D, E) qRT-PCR analysis of MMP1 expression in RUNX2 knockdown MDA-MB-231-Re and MDA-MB-231 cells (D) as well as the SUM-149 with RUNX2 overexpression (E). (F) RUNX2 and MMP1 expression of tumor sections from each group were determined by immunohistochemistry. Scale bar =50 µm. *P

Application: IHC    Species: Mouse    Sample:

Figure 8 MMP1 and RUNX2 showed positively correlation in relative mRNA and protein expression. (A) qRT-PCR analysis of the expression of RUNX2 and MMP1 in various breast cancer cell lines. (B, C) qRT-PCR and western-blotting analyses of RUNX2 (B) and MMP1 (C) expression in MDA-MB-231-Re cells or MDA-MB-231-Pa cells. (D, E) qRT-PCR analysis of MMP1 expression in RUNX2 knockdown MDA-MB-231-Re and MDA-MB-231 cells (D) as well as the SUM-149 with RUNX2 overexpression (E). (F) RUNX2 and MMP1 expression of tumor sections from each group were determined by immunohistochemistry. Scale bar =50 µm. *P

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