TCF4 Antibody - #DF6275

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製品: TCF4 Antibody
カタログ: DF6275
タンパク質の説明: Rabbit polyclonal antibody to TCF4
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 71kDa; 71kD(Calculated).
ユニプロット: P15884
RRID: AB_2838241

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
TCF4 Antibody detects endogenous levels of total TCF4.
RRID:
AB_2838241
引用形式: Affinity Biosciences Cat# DF6275, RRID:AB_2838241.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

bHLHb19; Class B basic helix-loop-helix protein 19; E2 2; FECD3; Immunoglobulin transcription factor 2; ITF 2; ITF-2; ITF2; ITF2_HUMAN; MGC149723; MGC149724; PTHS; SEF 2; SEF-2; SEF2 1; SEF2 1A; SEF2 1B; SEF2 1D; SEF2; SL3 3 enhancer factor 2; SL3-3 enhancer factor 2; TCF 4; TCF-4; TCF4; Transcription factor 4; Transcription factor 4, isoform C; Transcription factor 4, isoform D; Transcription factor 4, isoform E; Transcription factor 4, isoform L; Transcription factor 4, isoform M; Transcription factor 4, isoform R;

免疫原

免疫原:

A synthesized peptide derived from human TCF4, corresponding to a region within N-terminal amino acids.

Uniprot:

P15884(ITF2_HUMAN) >>Visit HPA database.

遺伝子(ID):
TCF4(6925)
発現特異性:
P15884 ITF2_HUMAN:

Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.

タンパク質の説明:
LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).
タンパク質配列:
MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDASNHMGQM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
60
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.

タンパク質ファミリー:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

参考文献

1). A Single-Cell RNA Sequencing Guided Multienzymatic Hydrogel Design for Self-Regenerative Repair in Diabetic Mandibular Defects. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39436107) [IF=27.4]
2). Regulation of endoplasmic reticulum stress and liver injury by hydroxysafflor yellow a through mir-222-3p-pten-wnt axis. European journal of pharmacology, 2025 (PubMed: 40675360) [IF=4.2]
3). MicroRNA-32-5p promotes the proliferation and metastasis of gastric cancer cells. Scientific reports, 2025 (PubMed: 39833289) [IF=3.8]
4). miR-140-y targets TCF4 to regulate the Wnt signaling pathway and promote embryonic feather follicle development in Hungarian white goose. Poultry science, 2024 (PubMed: 38350393) [IF=3.8]

Application: WB    Species: goose    Sample: GEDFs

Figure 6. miR-140-y directly regulates target gene TCF4. (A) RNAhybrid analysis demonstrates the putative target site between miR-140-y and TCF4 mRNA 3′-UTR. (B) Binding site and mutation site of miR-140-y and TCF4. (C) Luciferase assay in GEDFs cells co-transfected with pMir-TCF4 -3′-UTR-WT and miR-140-y mimics and pMir-TCF4 3′-UTR MT and miR-140-y mimics. (D) Changes in the expression level of miR-140-y in GEDFs after transfection with miR-140-y mimics and inhibitors. (E) Overexpression of miR-140-y can reduce the mRNA expression level of TCF4 in GEDFs cells; knockdown of miR-140-y increases the mRNA expression level of TCF4 in GEDFs cells. (F) Western blot of TCF4 in GEDFs after transfection with miR-140-y mimics and inhibitors. (G) Overexpression of miR-140-y can reduce the protein expression level of TCF4 in GEDFs cells; knockdown of miR-140-y Low increases the protein expression level of TCF4 in GEDFs cells. The data were shown as mean ± SEM, n = 3, ** p < 0.01.
5). SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling. American journal of cancer research, 2020 (PubMed: 32509397) [IF=3.6]

Application: WB    Species: human    Sample:

Figure 3.| SLC25A18 negatively regulates genes expression related to aerobics glycolysis, cell proliferation, andWnt/β-catenin cascade. (B) Gene set enrichment analysis implied a negative relationship between highly expressed SLC25A18 and the enrichment of CTNNB1 target genes and Wnt/β-catenin Signaling (HALLMARK_ WNT_BETA_CATENIN_SIGNALING, REACTOME_SIGNALING_BY_WNT) in patients with CRC from the TCGA database, (C)which was also verified by western blotting. All experiments were conducted in triplicates.
6). Fucoidan suppresses proliferation and epithelial-mesenchymal transition process via Wnt/β-catenin signalling in hemangioma. Experimental dermatology, 2024 (PubMed: 38514926) [IF=3.5]
7). Role of Wnt/β-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome. International Journal of Clinical and Experimental Pathology, 2017 [IF=1.1]

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