CYR61/CCN1 Antibody - #DF6250

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製品: CYR61/CCN1 Antibody
カタログ: DF6250
タンパク質の説明: Rabbit polyclonal antibody to CYR61/CCN1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Rabbit
分子量: 42kDa(Observed); 42kD(Calculated).
ユニプロット: O00622
RRID: AB_2838216

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#DF6250-BP

CYR61/CCN1 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Rabbit(88%)
クローナリティ:
Polyclonal
特異性:
CYR61/CCN1 Antibody detects endogenous levels of total CYR61/CCN1.
RRID:
AB_2838216
引用形式: Affinity Biosciences Cat# DF6250, RRID:AB_2838216.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

3CH61; CCN 1; CCN family member 1; CYR 61; Cyr61; CYR61 protein; CYR61_HUMAN; Cysteine rich angiogenic inducer 61; Cysteine rich heparin binding protein 61; Cysteine-rich angiogenic inducer 61; GIG 1; GIG1; IBP-10; IGF-binding protein 10; Igfbp 10; IGFBP-10; Igfbp10; Insulin like growth factor binding protein 10; Insulin-like growth factor-binding protein 10; Protein CYR61; Protein GIG1;

免疫原

免疫原:

A synthesized peptide derived from human CYR61/CCN1, corresponding to a region within the internal amino acids.

Uniprot:

O00622(CCN1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CYR61(3491)
タンパク質の説明:
Cyr61 is a secreted heparin binding protein, encoded by a growth factor-inducible immediate-early gene, that associates with the extracellular matrix and connective tissue. Cyr61 is a member of a distinct family of angiogenic and vasculogenic regulators designated CCN proteins, which includes connective tissue growth factor (CTGF) and the mouse Cyr61 homolog, Fisp12. As an angiogenic inducer, Cyr61 binds to the cell surface receptor Integrin aV/b3, where it then stimulates cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Expression of Cyr61 is elevated during vessel growth, wound healing and chondrocyte differentiation. Cyr61 is also detected in a wide variety of tumors as it induces tumor growth and functions as a marker of tumor progression.
タンパク質配列:
MSSRIARALALVVTLLHLTRLALSTCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFNDIHKFRD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the CCN family.

参考文献

1). Downregulation of miR-142-5p promotes tumor metastasis through directly regulating CYR61 expression in gastric cancer. Gastric Cancer, 2019 (PubMed: 30178386) [IF=6.0]

Application: IHC    Species: human    Sample: gastric and GC tissues

Fig. 5| High expression of CYR61 was associated with prognosis in GC. a The expression of CYR61 in normal gastric tissues (upper) and GC tissues (lower) (left: magnification ×200; right: magnification ×400).

Application: WB    Species: human    Sample: BGC823 and SGC7901 cells， AGS cells

Fig. 3 |CYR61 is a novel target gene of miR-142-5p. c Left and middle, qRT-PCR and western blot showed that the expression of CYR61 was significantly decreased in miR-142-5p-stably transfected BGC823 and SGC7901 cells as compared with control cells. Right, qRT-PCR and western blot showed that the expression of CYR61 was significantly increased in miR142-5p-inhibitor-stably transfected AGS cells as compared with control cells. *P < 0.05, **P < 0.01, ***P < 0.001.
2). CYR61 is Involved in Neonatal Hypoxic-ischemic Brain Damage Via Modulating Astrocyte-mediated Neuroinflammation. Neuroscience, 2024 (PubMed: 38908506) [IF=2.9]
3). Silencing soluble epoxide hydrolase protects against myocardial ischemia-reperfusion injury through modulation of the YAP signaling pathway and macrophage polarization. Journal of molecular histology, 2026 (PubMed: 41872574) [IF=2.9]
4). Upregulation of Angiomotin-like 2 ameliorates experimental pulmonary arterial hypertension by inactivating YAP1 signaling. Journal of cardiovascular pharmacology, 2022 (PubMed: 39027975) [IF=2.6]

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