製品: NCSTN Antibody
カタログ: DF6242
タンパク質の説明: Rabbit polyclonal antibody to NCSTN
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 78kDa; 78kD(Calculated).
ユニプロット: Q92542
RRID: AB_2838208

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(100%), Dog(83%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
NCSTN Antibody detects endogenous levels of total NCSTN.
RRID:
AB_2838208
引用形式: Affinity Biosciences Cat# DF6242, RRID:AB_2838208.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Anterior pharynx defective 2; APH 2; APH2; ATAG1874; KIAA0253; Ncstn; NCT; NICA_HUMAN; Nicastrin; RP11 517F10.1; RP11517F101;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q92542 NICA_HUMAN:

Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676).

タンパク質の説明:
Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).
タンパク質配列:
MATAGGGSGADPGSRGLLRLLSFCVLLAGLCRGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAGLAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
83
Horse
83
Bovine
83
Sheep
83
Dog
83
Xenopus
83
Chicken
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92542 基板として

Site PTM Type Enzyme
N45 N-Glycosylation
N55 N-Glycosylation
K101 Ubiquitination
K110 Acetylation
K110 Ubiquitination
K112 Acetylation
K127 Ubiquitination
N187 N-Glycosylation
N264 N-Glycosylation
K349 Acetylation
K351 Ubiquitination
N387 N-Glycosylation
K403 Ubiquitination
S425 Phosphorylation
S426 Phosphorylation
R434 Methylation
N435 N-Glycosylation
S437 Phosphorylation O00141 (SGK1)
N464 N-Glycosylation
N506 N-Glycosylation
N530 N-Glycosylation
N562 N-Glycosylation
N573 N-Glycosylation
N580 N-Glycosylation
N612 N-Glycosylation
S632 Phosphorylation
S638 Phosphorylation
T647 Phosphorylation
T649 Phosphorylation
T672 Phosphorylation
T674 Phosphorylation
S708 Phosphorylation

研究背景

機能:

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.

PTMs:

N-glycosylated.

細胞の位置付け:

Membrane>Single-pass type I membrane protein. Cytoplasmic vesicle membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in brain (at protein level). Widely expressed.

サブユニット構造:

Component of the gamma-secretase complex. The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Interacts with PSEN1 and PSEN2.

タンパク質ファミリー:

Belongs to the nicastrin family.

研究領域

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

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