PPAR gamma Antibody - #DF6073

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製品: PPAR gamma Antibody
カタログ: DF6073
タンパク質の説明: Rabbit polyclonal antibody to PPAR gamma
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 58kDa; 58kD(Calculated).
ユニプロット: P37231
RRID: AB_2838041

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
PPAR gamma Antibody detects endogenous levels of total PPAR gamma.
RRID:
AB_2838041
引用形式: Affinity Biosciences Cat# DF6073, RRID:AB_2838041.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CIMT1; GLM1; NR1C3; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARG; PPARG_HUMAN; PPARG1; PPARG2; PPARgamma;

免疫原

免疫原:

A synthesized peptide derived from human PPAR gamma, corresponding to a region within the internal amino acids.

Uniprot:

P37231(PPARG_HUMAN) >>Visit HPA database.

遺伝子(ID):
PPARG(5468)
発現特異性:
P37231 PPARG_HUMAN:

Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

タンパク質の説明:
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).
タンパク質配列:
MGETLGDSPIDPESDSFTDTLSANISQEMTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
71
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).

(Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.

PTMs:

O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.

Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity).

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. NOCT enhances its nuclear translocation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

タンパク質ファミリー:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the nuclear hormone receptor family. NR1 subfamily.

研究領域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

参考文献

1). Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma. International journal of molecular sciences, 2022 (PubMed: 35628510) [IF=5.6]

Application: WB    Species: Human    Sample: NPC cells

Figure 3. Leptin deficiency decreases SREBP1 via upregulation of PPAR-γ in NPC cells. (A) Western blot analysis of PPAR-γ and SREBP1 in leptin-depleted NPC cells in the absence or presence of GW9662. (B,C) The levels of TG and cholesterol in the indicated NPC cells with GW9662 treatment were measured. All data were obtained from three independent experiments. Data are presented as the mean ± SD. *: p < 0.05; **: p < 0.01; ***: p < 0.001.
2). TMEM88 Modulates Lipid Synthesis and Metabolism Cytokine by Regulating Wnt/β-Catenin Signaling Pathway in Non-Alcoholic Fatty Liver Disease. Frontiers in pharmacology, 2022 (PubMed: 35058782) [IF=5.6]

Application: WB    Species: Mouse    Sample:

FIGURE 2 Expression level of lipid metabolism cytokine in MCD-fed mice. (A,B) The mRNA and protein expression levels of FASN and SERBP-1C were increased. PPAR-α and ACOX-1 expression were decreased in NAFLD liver tissues. (Data are represented by at least three independent mean ± SD, *p < 0.05, **p < 0.01 vs normal group).
3). E4BP4 mediates glucocorticoid-regulated adipogenesis through COX2. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2017 (PubMed: 28416324) [IF=3.8]

Application: WB    Species: mouse    Sample:

Fig. 3. E4BP4 mediates the actions of glucocorticoid in adipocyte formation. (a, b) The adipogenic phenotypes of 3T3-L1 cells transfected with pCDNA3.1-E4BP4 or control after induction by MI for 6 days were assessed by ORO staining. (b) Triglyceride accumulation was quantified and normalized to protein amount. (c) The mRNA levels of PPARg2, aP2, adiponectin, and LPL at day 6 were detected by qPCR. (d) The protein levels of PPARg and aP2 at day 6 were measured by Western blot. (e) The adipogenic phenotypes of 3T3-L1 cells transfected with siE4BP4 or control after being induced by DEX only for 6 days were assessed by ORO staining. (f) Triglyceride accumulation was quantified and normalized to protein amount. (g) qPCR analysis of PPARg2, aP2, adiponectin, and LPL. (h) Western blot analysis of PPARg and aP2. The results are expressed as mean ± SD (n ¼ 3); *P < 0.05 and **P < 0.01 indicate significant difference from the control.

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