FABP4 Antibody - #DF6035

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製品: FABP4 Antibody
カタログ: DF6035
タンパク質の説明: Rabbit polyclonal antibody to FABP4
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Horse, Rabbit, Dog
分子量: 15kDa; 15kD(Calculated).
ユニプロット: P15090
RRID: AB_2838009

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(83%), Rabbit(92%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
FABP4 Antibody detects endogenous levels of total FABP4.
RRID:
AB_2838009
引用形式: Affinity Biosciences Cat# DF6035, RRID:AB_2838009.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

3T3-L1 lipid-binding protein; 422/aP2; A-FABP; adipocyte; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; Adipocyte protein AP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBP; ALBP/Ap2; aP2; Epididymis secretory protein Li 104; FABP; FABP4; FABP4_HUMAN; Fatty acid binding protein 4 adipocyte; Fatty acid binding protein 4; Fatty acid binding protein adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein; HEL S 104; Lbpl; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422;

免疫原

免疫原:
Uniprot:

P15090(FABP4_HUMAN) >>Visit HPA database.

遺伝子(ID):
FABP4(2167)
タンパク質の説明:
Fatty acid binding proteins (FABPs) bind to fatty acids and other lipids to function as cytoplasmic lipid chaperones (1). They participate in the transport of fatty acids and other lipids to various cellular pathways (2). The predominant fatty acid binding protein found in adipocytes is FABP4, also called adipocyte fatty acid binding protein or aP2. FABP4 is also expressed in macrophages (3). FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes (4). Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE (3). Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders (1).
タンパク質配列:
MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Dog
92
Horse
83
Bovine
75
Xenopus
58
Pig
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15090 基板として

Site PTM Type Enzyme
C2 Acetylation
T8 Phosphorylation
Y20 Phosphorylation P06213 (INSR)
K101 Acetylation
T103 Phosphorylation
T104 Phosphorylation
Y129 Phosphorylation

研究背景

機能:

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Monomer. Homodimer. Interacts with PPARG (By similarity).

タンパク質ファミリー:

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

研究領域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

参考文献

1). IL-17A promotes fatty acid uptake through the IL-17A/IL-17RA/p-STAT3/FABP4 axis to fuel ovarian cancer growth in an adipocyte-rich microenvironment. CANCER IMMUNOLOGY IMMUNOTHERAPY (PubMed: 31802182) [IF=5.8]

Application: WB    Species: Mouse    Sample: OvCa cells

Fig. 1 rhIL-17A increased FABP4 expression in OvCa cells via STAT3 signaling. Dose–efect (a, b) and time–efect (c) experiments were performed in A2780 and OVCAR3 cells. a mRNA level of FABP4 after rhIL-17A treatment. b, c Protein expression of FABP4 after rhIL-17A treatment. d-(a) Protein expression of FABP4, p-STAT3 and STAT3 after rhIL-17A and/or STATTIC treatment (A2780: 0.3125  μM; OVCAR3: 1.25  μM). d-(b) The relative expression of proteins in d-(a). Three independent experiments were performed and a representative image is shown. Data represent the mean±SD from three independent experiments. *p<0.05, **p<0.01

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