PRF1 Antibody - #DF6004
| 製品: | PRF1 Antibody |
| カタログ: | DF6004 |
| タンパク質の説明: | Rabbit polyclonal antibody to PRF1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Horse, Rabbit, Dog |
| 分子量: | 61kDa; 61kD(Calculated). |
| ユニプロット: | P14222 |
| RRID: | AB_2837981 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6004, RRID:AB_2837981.
折りたたみ／展開
Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; P1; PERF_HUMAN; perforin 1 (pore forming protein); Perforin 1; Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF; Pore forming protein; prf1; SHGC-10760;
免疫原
- P14222 PERF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14222 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S112 | Phosphorylation | Uniprot | |
| N205 | N-Glycosylation | Uniprot | |
| T533 | Phosphorylation | Uniprot |
研究背景
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
N-glycosylated.
Cytoplasmic granule lumen. Secreted. Cell membrane>Multi-pass membrane protein. Endosome lumen.
Note: Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
Monomer, as soluble protein. Homooligomer. Oligomerization is required for pore formation.
The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
Belongs to the complement C6/C7/C8/C9 family.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.
· Human Diseases > Immune diseases > Autoimmune thyroid disease.
· Human Diseases > Immune diseases > Allograft rejection.
· Human Diseases > Immune diseases > Graft-versus-host disease.
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
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