FOXP2 Antibody - #AF5385

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製品: FOXP2 Antibody
カタログ: AF5385
タンパク質の説明: Rabbit polyclonal antibody to FOXP2
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken
分子量: 79.9 kDa; 80kD(Calculated).
ユニプロット: O15409
RRID: AB_2837870

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 100ul $280 在庫あり
 200ul $350 在庫あり

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
FOXP2 Antibody detects endogenous levels of total FOXP2.
RRID:
AB_2837870
引用形式: Affinity Biosciences Cat# AF5385, RRID:AB_2837870.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CAG repeat protein 44; CAGH44; DKFZp686H1726; Forkhead box P2; Forkhead box protein P2; forkhead/winged-helix transcription factor; FOX P2; FOXP2; FOXP2_HUMAN; HGNC11222; HGNC11956; SPCH 1; SPCH1; TNRC 10; TNRC10; trinucleotide repeat containing 10; Trinucleotide repeat containing gene 10 protein; Trinucleotide repeat-containing gene 10 protein;

免疫原

免疫原:

A synthesized peptide derived from human FOXP2, corresponding to a region within C-terminal amino acids.

Uniprot:

O15409(FOXP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
FOXP2(93986)
発現特異性:
O15409 FOXP2_HUMAN:

Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.

タンパク質の説明:
Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
タンパク質配列:
MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEVSTVELLHLQQQQALQAARQLLLQQQTSGLKSPKSSDKQRPLQVPVSVAMMTPQVITPQQMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYKKQQEQLHLQLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQLAAQQLVFQQQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGVHSMEDNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHEETGASHTLYGHGVCKWPGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLEIQLSKERERLQAMMTHLHMRPSEPKPSPKPLNLVSSVTMSKNMLETSPQSLPQTPTTPTAPVTPITQGPSVITPASVPNVGAIRRRHSDKYNIPMSSEIAPNYEFYKNADVRPPFTYATLIRQAIMESSDRQLTLNEIYSWFTRTFAYFRRNAATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEVEYQKRRSQKITGSPTLVKNIPTSLGYGAALNASLQAALAESSLPLLSNPGLINNASSGLLQAVHEDLNGSLDHIDSNGNSSPGCSPQPHIHSIHVKEEPVIAEDEDCPMSLVTTANHSPELEDDREIEEEPLSEDLE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
70
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Plays a role in synapse formation by regulating SRPX2 levels. Involved in neural mechanisms mediating the development of speech and language.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.

タンパク質ファミリー:

The leucine-zipper is required for dimerization and transcriptional repression.

参考文献

1). RGMa Participates in the Blood–Brain Barrier Dysfunction Through BMP/BMPR/YAP Signaling in Multiple Sclerosis. Frontiers in Immunology, 2022 (PubMed: 35664003) [IF=5.7]
2). GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion. BMC biology, 2024 (PubMed: 38702712) [IF=5.4]

Application: WB    Species: Rat    Sample:

Fig. 2 Highly expressed FOXP2 after stimulation by GnRH promotes gonadotropin synthesis and secretion. A Immunohistochemical detection of FOXP2 protein levels in rat adenohypophysis after GnRH treatment. Positive signal regions of FOXP2 protein were analyzed using ImageJ (n = 3). B-D RT-qPCR analysis Foxp2 mRNA expression after GnRH treatment in rat adenohypophysis tissues (B, n = 3), primary rat adenohypophysis cells (C, n = 3) and LβT2 cells (D, n = 3). E Correlation analysis of Foxp2 mRNA expression and Fshb (left)/Lhb (right) mRNA expression in rats (n = 20). F Correlation analysis of Foxp2 mRNA expression and FSH (left)/LH (right) secretion level in rats (n = 20). G WB analysis FOXP2 protein expression after GnRH treatment in LβT2 cells and primary rat adenohypophysis cells (n = 3). H–K RT-qPCR analysis Fshb and Lhb mRNA expression transfected with siNC (siNC group) or FOXP2 siRNA (siFOXP2 group) in LβT2 cells (H, n = 3) and primary rat adenohypophysis cells (J, n = 3), respectively. ELISA analysis secretion levels of FSH and LH transfected with siNC (siNC group) or FOXP2 siRNA (siFOXP2 group) in LβT2 cells (I, n = 5) and primary rat adenohypophysis cells (K, n = 5), respectively. L-O RT-qPCR analysis Fshb and Lhb mRNA expression transfected with vector or FOXP2 overexpression plasmid in LβT2 cells (L, n = 3) and primary rat adenohypophysis cells (N, n = 3), respectively. ELISA analysis secretion levels of FSH and LH transfected with vector (Vector group) and FOXP2 overexpression plasmid (FOXP2 group) in LβT2 cells (M, n = 5) and primary rat adenohypophysis cells (O, n = 5), respectively. *, P 

Application: IHC    Species: Rat    Sample:

Fig. 2 Highly expressed FOXP2 after stimulation by GnRH promotes gonadotropin synthesis and secretion. A Immunohistochemical detection of FOXP2 protein levels in rat adenohypophysis after GnRH treatment. Positive signal regions of FOXP2 protein were analyzed using ImageJ (n = 3). B-D RT-qPCR analysis Foxp2 mRNA expression after GnRH treatment in rat adenohypophysis tissues (B, n = 3), primary rat adenohypophysis cells (C, n = 3) and LβT2 cells (D, n = 3). E Correlation analysis of Foxp2 mRNA expression and Fshb (left)/Lhb (right) mRNA expression in rats (n = 20). F Correlation analysis of Foxp2 mRNA expression and FSH (left)/LH (right) secretion level in rats (n = 20). G WB analysis FOXP2 protein expression after GnRH treatment in LβT2 cells and primary rat adenohypophysis cells (n = 3). H–K RT-qPCR analysis Fshb and Lhb mRNA expression transfected with siNC (siNC group) or FOXP2 siRNA (siFOXP2 group) in LβT2 cells (H, n = 3) and primary rat adenohypophysis cells (J, n = 3), respectively. ELISA analysis secretion levels of FSH and LH transfected with siNC (siNC group) or FOXP2 siRNA (siFOXP2 group) in LβT2 cells (I, n = 5) and primary rat adenohypophysis cells (K, n = 5), respectively. L-O RT-qPCR analysis Fshb and Lhb mRNA expression transfected with vector or FOXP2 overexpression plasmid in LβT2 cells (L, n = 3) and primary rat adenohypophysis cells (N, n = 3), respectively. ELISA analysis secretion levels of FSH and LH transfected with vector (Vector group) and FOXP2 overexpression plasmid (FOXP2 group) in LβT2 cells (M, n = 5) and primary rat adenohypophysis cells (O, n = 5), respectively. *, P 

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