Transferrin Receptor Antibody - #AF5343

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製品: Transferrin Receptor Antibody
カタログ: AF5343
タンパク質の説明: Rabbit polyclonal antibody to Transferrin Receptor
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
反応性: Human, Mouse, Monkey
予測: Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 50kD(reduced),80~130kDa; 85kD(Calculated).
ユニプロット: P02786
RRID: AB_2837828

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Monkey
予測:
Rat(%), Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%)
クローナリティ:
Polyclonal
特異性:
Transferrin Receptor Antibody detects endogenous levels of total Transferrin Receptor.
RRID:
AB_2837828
引用形式: Affinity Biosciences Cat# AF5343, RRID:AB_2837828.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD 71; CD71; CD71 antigen; IMD46; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; p90; sTfR; T9; TFR 1; TfR; TfR1; TFR1_HUMAN; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin receptor protein 1, serum form; Trfr;

免疫原

免疫原:

A synthesized peptide derived from human Transferrin Receptor

Uniprot:

P02786(TFR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
TFRC(7037)
タンパク質配列:
MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
89
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake.

(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

PTMs:

N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.

Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).

Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

細胞の位置付け:

Cell membrane>Single-pass type II membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the peptidase M28 family. M28B subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

参考文献

1). A cancer-specific activatable theranostic nanodrug for enhanced therapeutic efficacy via amplification of oxidative stress. Theranostics, 2020 (PubMed: 31903126) [IF=12.4]
2). Quercetin alleviates LPS/iE-DAP-induced liver injury by suppressing ferroptosis via regulating ferritinophagy and intracellular iron efflux. Redox biology, 2025 (PubMed: 39986118) [IF=10.7]
3). Defective ferritinophagy and imbalanced iron metabolism in PBDE-47-triggered neuronal ferroptosis and salvage by Canolol. The Science of the total environment, 2024 (PubMed: 38750757) [IF=8.2]
4). Allogeneic platelet-rich plasma inhibits ferroptosis in promoting wound repair of type 2 diabetic ulcers. Free radical biology & medicine, 2024 (PubMed: 38408545) [IF=7.1]
5). Activation of AMPKα2 attenuated doxorubicin-induced cardiotoxicity via inhibiting lipid peroxidation associated ferroptosis. Free Radical Biology and Medicine, 2023 (PubMed: 37331642) [IF=7.1]
6). GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract. Free Radical Biology and Medicine, 2023 (PubMed: 37156294) [IF=7.1]
7). Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37224754) [IF=6.9]

Application: WB    Species: Mouse    Sample: renal tissues

Fig. 4. The characteristic changes of ferroptosis were rescued by TUDCA treatment in DN mice. The qRT-PCR results of SLC7A11 and TFR-1 in each group (n = 3) (A). The WB results of SLC7A11 and TFR-1 among groups (n = 3) (B - C). Immunohistochemistry and quantitative analysis of SLC7A11 and TFR-1 in NC, DN, and TUDCA group (n = 3) (D - E). The content of iron (F), MDA (G), and GSH (H) in renal tissues (n = 6). Data are expressed as the mean ± SEM.

Application: IHC    Species: Mouse    Sample: renal tissues

Fig. 4. The characteristic changes of ferroptosis were rescued by TUDCA treatment in DN mice. The qRT-PCR results of SLC7A11 and TFR-1 in each group (n = 3) (A). The WB results of SLC7A11 and TFR-1 among groups (n = 3) (B - C). Immunohistochemistry and quantitative analysis of SLC7A11 and TFR-1 in NC, DN, and TUDCA group (n = 3) (D - E). The content of iron (F), MDA (G), and GSH (H) in renal tissues (n = 6). Data are expressed as the mean ± SEM.
8). Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy, 2023 (PubMed: 36379122) [IF=6.9]
9). Magnetic graphene oxide nanocomposites induce cytotoxicity in ADSCs via GPX4 regulating ferroptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2024 [IF=6.2]
10). Fine particulate matter potentiates Th17-cell pathogenicity in experimental autoimmune uveitis via ferroptosis. Ecotoxicology and environmental safety, 2024 (PubMed: 39232294) [IF=6.2]

Application: WB    Species: Mouse    Sample:

Fig. 5. PM2.5 induced iron overload and lipid peroxidation in EAU. (A) Representative fluorescence images (left) and fluorescence intensity quantification (right) of FerroOrange indicating the intracellular Fe2+ content of CD4+ T cells in EUA mice with or without PM2.5 exposure (n=6/ group; unpaired student’s t-test; scale bar: 25μm). (B) Representative flow cytometry images (left) and quantification (right) of ROS level in CD4+ T cells of EUA mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (C) Representative flow cytometry images (left) and quantification (right) of oxidized and non-oxidized lipid production of CD4+ T cells in EUA mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (D) The concentration of MDA in serum of EAU mice with or without PM2.5 exposure (n=3/ group; unpaired student’s t-test). (E) The concentration of MDA in CD4+ T cells of EAU mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (F) GSH/GSSH ratio in CD4+ T cells of EUA mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (G) Representative Western blotting images of TFRC, FTH1, FTL1, ACSL1, HMOX1, GPX4 and β-actin in vivo (left) and in vitro (right). (H) Quantification of relative expression of TFRC, FTH1, FTL1, ACSL1, HMOX1, GPX4 in vivo and in vitro (n=3/ group; one-way ANOVA). β-actin served as an internal control. Data represent mean ± SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001.
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