LEG7 Antibody - #AF5300

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製品: LEG7 Antibody
カタログ: AF5300
タンパク質の説明: Rabbit polyclonal antibody to LEG7
アプリケーション: IHC
Cited expt.:
反応性: Human, Mouse, Rat
分子量: 15 KD(Observed); 15kD(Calculated).
ユニプロット: P47929
RRID: AB_2837785

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#AF5300-BP

LEG7 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
クローナリティ:
Polyclonal
特異性:
LEG7 Antibody detects endogenous levels of total LEG7.
RRID:
AB_2837785
引用形式: Affinity Biosciences Cat# AF5300, RRID:AB_2837785.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Gal-7; GAL7; Galectin 7B; Galectin-7; HKL-14; Human keratinocyte lectin 14; Keratinocyte lectin 14; Lectin galactoside binding soluble 7; Lectin, galactoside binding, soluble, 7B; LEG7_HUMAN; LGALS7; LGALS7A; LGALS7B; P53 induced protein 1; p53-induced gene 1 protein; Pi7; PIG1; TP53I1;

免疫原

免疫原:

A synthesized peptide derived from human LEG7, corresponding to a region within the internal amino acids.

Uniprot:

P47929(LEG7_HUMAN) >>Visit HPA database.

遺伝子(ID):
LGALS7; LGALS7B(3963)
発現特異性:
P47929 LEG7_HUMAN:

Mainly expressed in stratified squamous epithelium.

タンパク質の説明:
Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
タンパク質配列:
MSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF

研究背景

機能:

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

細胞の位置付け:

Cytoplasm. Nucleus. Secreted.
Note: May be secreted by a non-classical secretory pathway.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in stratified squamous epithelium.

参考文献

1). CRLF1 is a key regulator in the ligamentum flavum hypertrophy. Frontiers in Cell and Developmental Biology, 2020 (PubMed: 33072735) [IF=4.6]

Application: WB    Species: human    Sample: LF cells

FIGURE 6 | CRLF1 plays a crucial role in LF fibrosis. (A) CRLF1 expression induced by TGF-β1 in LF cells transfected with siRNAs. (B,C) ERK signaling pathway activation by CRLF1 and TGF-β1. (D) siCRLF1 attenuated the ERK phosphorylation induced by TGF-β1. (E) siCRLF1 reduced the fibrosis markers expression induced by TGF-β1. (F,G) Inhibition of the ERK pathway attenuated the CRLF1- and TGF-β1-induced expressions of fibrosis markers in LF cells. (H) siCRLF1 significantly reduced the pro-fibrotic effect of IL-1β (20 ng/ml). (I) siCRLF1 significantly reduced the pro-fibrotic effect of mechanical stretching forces.

Application: WB    Species: human    Sample: LF cells

FIGURE 4 | CRLF1 stimulates the cell matrix at the post-transcriptional level. LF cells were exposed to CRLF1 for 24 h. (A) Cell lysates were obtained for Western blot analysis.

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