CD69 Antibody - #AF5275

Figure 1. TRM markers increased in the lungs of mice with pulmonary fibrosis. A: BLM was administered by intratracheal instillation at a single dose of 2 U/Kg to induce a pulmonary fibrosis model in male C57BL/6J mice. After 21 days, the mice were euthanized. Created with BioRender.com. B: body weight (left gray) and lung weight (right blue) of PBS- and BLM-treated mice (n = 4). C: representative photomicrographs of HE staining, Masson trichrome staining (collagen fibers shown in blue), and Sirius red staining (collagen fibers shown in red) of lung sections. Scale bars: 50 µm. D: collagen deposition area (%) of lung sections in each group (n = 4). E and F: representative gross photographs of lungs and western blots of collagen-I, α-SMA, CD69, and CD103 in lung tissues (n = 4). G: mRNA levels of Col1a1 in the lung (n = 4). H: representative immunofluorescence images of CD69 and CD103 in lung. Scale bars: 50 µm. I: the quantification histograms of CD69 and CD103 staining in lung (n = 4). B, D, F, G, and I: data are shown as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. B, D, F, and I: one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. G: two-tailed unpaired Student’s t test. α-SMA, alpha-smooth muscle actin; BLM, bleomycin.

Figure 1. TRM markers increased in the lungs of mice with pulmonary fibrosis. A: BLM was administered by intratracheal instillation at a single dose of 2 U/Kg to induce a pulmonary fibrosis model in male C57BL/6J mice. After 21 days, the mice were euthanized. Created with BioRender.com. B: body weight (left gray) and lung weight (right blue) of PBS- and BLM-treated mice (n = 4). C: representative photomicrographs of HE staining, Masson trichrome staining (collagen fibers shown in blue), and Sirius red staining (collagen fibers shown in red) of lung sections. Scale bars: 50 µm. D: collagen deposition area (%) of lung sections in each group (n = 4). E and F: representative gross photographs of lungs and western blots of collagen-I, α-SMA, CD69, and CD103 in lung tissues (n = 4). G: mRNA levels of Col1a1 in the lung (n = 4). H: representative immunofluorescence images of CD69 and CD103 in lung. Scale bars: 50 µm. I: the quantification histograms of CD69 and CD103 staining in lung (n = 4). B, D, F, G, and I: data are shown as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. B, D, F, and I: one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. G: two-tailed unpaired Student’s t test. α-SMA, alpha-smooth muscle actin; BLM, bleomycin.