製品: hnRNP G Antibody
カタログ: AF5263
タンパク質の説明: Rabbit polyclonal antibody to hnRNP G
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 42 kDa; 42kD(Calculated).
ユニプロット: P38159
RRID: AB_2837749

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
hnRNP G Antibody detects endogenous levels of total hnRNP G.
RRID:
AB_2837749
引用形式: Affinity Biosciences Cat# AF5263, RRID:AB_2837749.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G; HNRNPG; HNRPG; RBMX; RBMXP1; RBMXRT; RNA binding motif protein, X chromosome; RNA binding motif protein, X linked; RNMX;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P38159 RBMX_HUMAN:

Expressed strongly in oral keratinocytes, but only weakly detected in oral squamous cell carcinomas (at protein level).

タンパク質の説明:
Heterogeneous nuclear ribonuclearproteins (hnRNPs) are nucleic acid binding proteins and have critical roles in DNA repair, telomere regulation, and transcriptional gene regulation. hnRNP G has tumor suppressive activity and this RNA binding protein which may be involved in pre-mRNA splicing.
タンパク質配列:
MVEADRPGKLFIGGLNTETNEKALEAVFGKYGRIVEVLLMKDRETNKSRGFAFVTFESPADAKDAARDMNGKSLDGKAIKVEQATKPSFESGRRGPPPPPRSRGPPRGLRGGRGGSGGTRGPPSRGGHMDDGGYSMNFNMSSSRGPLPVKRGPPPRSGGPPPKRSAPSGPVRSSSGMGGRAPVSRGRDSYGGPPRREPLPSRRDVYLSPRDDGYSTKDSYSSRDYPSSRDTRDYAPPPRDYTYRDYGHSSSRDDYPSRGYSDRDGYGRDRDYSDHPSGGSYRDSYESYGNSRSAPPTRGPPPSYGGSSRYDDYSSSRDGYGGSRDSYSSSRSDLYSSGRDRVGRQERGLPPSMERGYPPPRDSYSSSSRGAPRGGGRGGSRSDRGGGRSRY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38159 基板として

Site PTM Type Enzyme
K9 Acetylation
K9 Ubiquitination
T17 Phosphorylation
K22 Acetylation
K22 Ubiquitination
K30 Acetylation
K30 Ubiquitination
K41 Ubiquitination
R49 Methylation
T55 Phosphorylation
S58 Phosphorylation
K63 Acetylation
K63 Sumoylation
K63 Ubiquitination
K72 Acetylation
S73 Phosphorylation
K77 Ubiquitination
K80 Acetylation
K80 Sumoylation
T85 Phosphorylation
K86 Acetylation
K86 Ubiquitination
S88 Phosphorylation
S91 Phosphorylation
R94 Methylation
R101 Methylation
S102 Phosphorylation
R103 Methylation
R107 Methylation
R110 Methylation
R113 Methylation
S116 Phosphorylation
S124 Phosphorylation
R125 Methylation
Y134 Phosphorylation
S135 Phosphorylation
S141 Phosphorylation
S142 Phosphorylation
S143 Phosphorylation
R144 Methylation
K150 Acetylation
K150 Methylation
K150 Ubiquitination
R151 Methylation
R156 Methylation
S157 Phosphorylation
K163 Acetylation
R164 Methylation
S165 Phosphorylation
S168 Phosphorylation
R172 Methylation
S174 Phosphorylation
S175 Phosphorylation
R180 Methylation
R185 Methylation
R187 Methylation
S189 Phosphorylation
Y190 Phosphorylation
Y206 Phosphorylation
S208 Phosphorylation
Y214 Phosphorylation
S215 Phosphorylation
T216 Phosphorylation
K217 Acetylation
K217 Ubiquitination
S219 Phosphorylation
Y220 Phosphorylation
S221 Phosphorylation
S222 Phosphorylation
Y225 Phosphorylation
T231 Phosphorylation
Y234 Phosphorylation
Y241 Phosphorylation
T242 Phosphorylation
Y243 Phosphorylation
Y246 Phosphorylation
S249 Phosphorylation
S250 Phosphorylation
S251 Phosphorylation
Y255 Phosphorylation
S257 Phosphorylation
R258 Methylation
Y260 Phosphorylation
S261 Phosphorylation
R263 Methylation
Y266 Phosphorylation
Y272 Phosphorylation
S273 Phosphorylation
S277 Phosphorylation
S280 Phosphorylation
Y281 Phosphorylation
S284 Phosphorylation
Y285 Phosphorylation
S287 Phosphorylation
Y288 Phosphorylation
S291 Phosphorylation
R292 Methylation
S293 Phosphorylation
R298 Methylation
S303 Phosphorylation
Y304 Phosphorylation
S307 Phosphorylation
S308 Phosphorylation
Y310 Phosphorylation
Y313 Phosphorylation
S314 Phosphorylation
S315 Phosphorylation
S316 Phosphorylation
R317 Methylation
Y320 Phosphorylation
S323 Phosphorylation
S326 Phosphorylation Q96GD4 (AURKB)
Y327 Phosphorylation
S328 Phosphorylation
S329 Phosphorylation
S330 Phosphorylation
S332 Phosphorylation
Y335 Phosphorylation
S336 Phosphorylation
S337 Phosphorylation
R339 Methylation
R347 Methylation
S352 Phosphorylation
R355 Methylation
R369 Methylation
R373 Methylation
R377 Methylation
S380 Phosphorylation
R381 Methylation

研究背景

機能:

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.

PTMs:

O-glycosylated.

Arg-185 is dimethylated, probably to asymmetric dimethylarginine.

Cleavage of initiator Met is partial. If Met-1 is not removed, it is acetylated. If it is removed, Val-2 is acetylated.

細胞の位置付け:

Nucleus.
Note: Component of ribonucleosomes. Localizes in numerous small granules in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed strongly in oral keratinocytes, but only weakly detected in oral squamous cell carcinomas (at protein level).

サブユニット構造:

Homomultimer. Interacts with SAFB/SAFB1 (By similarity). Found in the supraspliceosome complex. Identified in the spliceosome C complex. Interacts with KHDRBS3. Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, NCOA5 and PPP1CA. Interacts with CLK2, KHDRBS2, SAFB, TRA2B and YTHDC1. Interacts with ERAP1; the interaction is RNA-independent.

タンパク質ファミリー:

The RRM domain is necessary for RNA-binding, but not for splice site selection, indicating that its splicing activity does not require direct binding to RNA.

研究領域

· Genetic Information Processing > Transcription > Spliceosome.

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