製品: Integrin alpha 5 Antibody
カタログ: AF5200
タンパク質の説明: Rabbit polyclonal antibody to Integrin alpha 5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Horse, Rabbit, Dog
分子量: 114kDa,150kDa; 115kD(Calculated).
ユニプロット: P08648
RRID: AB_2837686

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Integrin alpha 5 Antibody detects endogenous levels of total Integrin alpha 5.
RRID:
AB_2837686
引用形式: Affinity Biosciences Cat# AF5200, RRID:AB_2837686.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; Fibronectin receptor, alpha subunit; FNRA; Integrin alpha 5 (fibronectin receptor alpha); Integrin alpha-5; Integrin alpha-5 light chain; Integrin alpha-F; Integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITA5_HUMAN; Itga5; Very late activation protein 5, alpha subunit; VLA-5; VLA5; VLA5A;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and Vitronectin.
タンパク質配列:
MGSRTPESPLHAVQLRWGPRRRPPLLPLLLLLLPPPPRVGGFNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Xenopus
73
Zebrafish
62
Pig
0
Bovine
0
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08648 基板として

Site PTM Type Enzyme
N84 N-Glycosylation
S123 Phosphorylation
S124 Phosphorylation
S126 Phosphorylation
S127 Phosphorylation
S128 Phosphorylation
K137 Ubiquitination
K166 Ubiquitination
N182 N-Glycosylation
S210 Phosphorylation
N297 N-Glycosylation
N307 N-Glycosylation
N316 N-Glycosylation
S394 Phosphorylation
S395 Phosphorylation
Y487 Phosphorylation
N609 N-Glycosylation
N675 N-Glycosylation
C731 S-Nitrosylation
S742 Phosphorylation
N773 N-Glycosylation
S887 Phosphorylation
K943 Ubiquitination
K1042 Ubiquitination
S1047 Phosphorylation

研究背景

機能:

Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling.

(Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.

(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.

(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

PTMs:

Proteolytic cleavage by PCSK5 mediates activation of the precursor.

細胞の位置付け:

Membrane>Single-pass type I membrane protein. Cell junction>Focal adhesion. Cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with CIB1. ITGA5:ITGB1 interacts with CCN3. ITGA5:ITGB1 interacts with FBN1. ITGA5:ITGB1 interacts with IL1B.

(Microbial infection) Integrin ITGA5:ITGB1 interacts with human metapneumovirus fusion protein.

(Microbial infection) Integrin ITGA2:ITGB1 interacts with human parvovirus B19 capsid proteins.

(Microbial infection) Interacts with HIV-1 Tat.

タンパク質ファミリー:

Belongs to the integrin alpha chain family.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

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