S100A10 Antibody - #AF5180

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製品: S100A10 Antibody
カタログ: AF5180
タンパク質の説明: Rabbit polyclonal antibody to S100A10
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 11 kDa(Observed); 11kD(Calculated).
ユニプロット: P60903
RRID: AB_2837666

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#AF5180-BP

S100A10 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
S100A10 Antibody detects endogenous levels of total S100A10.
RRID:
AB_2837666
引用形式: Affinity Biosciences Cat# AF5180, RRID:AB_2837666.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

42C; AA409961; AL024248; Annexin II ligand; Annexin II ligand, calpactin I, light polypeptide; Annexin II tetramer (AIIt) p11 subunit; Annexin II, light chain; ANX2L; ANX2LG; Ca[1]; CAL12; CAL1L; Calpactin I light chain; Calpactin I, p11 subunit; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11; GP11; MGC111133; Nerve growth factor-induced protein 42C; OTTHUMP00000015269; OTTHUMP00000015270; p10; p10 protein; p11; Protein S100 A10; Protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)); S100 calcium binding protein A10 (calpactin); S100 calcium binding protein A10; S100 calcium-binding protein A10; S100a10; S10AA_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human S100A10, corresponding to a region within the internal amino acids.

Uniprot:

P60903(S10AA_HUMAN) >>Visit HPA database.

遺伝子(ID):
S100A10(6281)
タンパク質の説明:
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
タンパク質配列:
MPSQMEHAMETMMFTFHKFAGDKGYLTKEDLRVLMEKEFPGFLENQKDPLAVDKIMKDLDQCRDGKVGFQSFFSLIAGLTIACNDYFVVHMKQKGKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
77
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

タンパク質ファミリー:

Belongs to the S-100 family.

参考文献

1). Notch signaling activation contributes to paclitaxel-induced neuropathic pain via activation of A1 astrocytes. European journal of pharmacology, 2022 (PubMed: 35777441) [IF=4.2]

Application: WB    Species: Rat    Sample: spinal cord

Fig. 2. Astrocytes were activated as the A1 phenotype in spinal cord. (A–E) The spinal expression of GFAP, C3 and Serping1 were significantly increased in spinal cord at 14 d and 21 d after intraperitoneal injection of paclitaxel, while the spinal S100A10 and PTX3 were decreased at 14 d and 21 d (*p < 0.05, **p < 0.01, ***p < 0.001 compared with the vehicle group, n=6 in each group). (F–G) Dual-label immunofluorescence showed that A1 astrocytes markers (C3, Serping1) and A2 astrocytes markers (S100A10, PTX3) were mostly colocalized with GFAP in the dorsal horn of spinal cord. Scale Bar: 50 μm and 100 μm, respectively (n=4). The white arrow indicated typical co-staining cells.

Application: IF/ICC    Species: Rat    Sample: spinal cord

Fig. 2. Astrocytes were activated as the A1 phenotype in spinal cord. (A–E) The spinal expression of GFAP, C3 and Serping1 were significantly increased in spinal cord at 14 d and 21 d after intraperitoneal injection of paclitaxel, while the spinal S100A10 and PTX3 were decreased at 14 d and 21 d (*p < 0.05, **p < 0.01, ***p < 0.001 compared with the vehicle group, n=6 in each group). (F–G) Dual-label immunofluorescence showed that A1 astrocytes markers (C3, Serping1) and A2 astrocytes markers (S100A10, PTX3) were mostly colocalized with GFAP in the dorsal horn of spinal cord. Scale Bar: 50 μm and 100 μm, respectively (n=4). The white arrow indicated typical co-staining cells.

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