Connexin 45 / GJA7 Antibody - #AF5108

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製品: Connexin 45 / GJA7 Antibody
カタログ: AF5108
タンパク質の説明: Rabbit polyclonal antibody to Connexin 45 / GJA7
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 45 kDa(Observed); 45kD(Calculated).
ユニプロット: P36383
RRID: AB_2837594

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#AF5108-BP

Connexin 45 / GJA7 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(89%), Horse(89%), Rabbit(89%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Connexin 45 / GJA7 Antibody detects endogenous levels of total Connexin 45 / GJA7.
RRID:
AB_2837594
引用形式: Affinity Biosciences Cat# AF5108, RRID:AB_2837594.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Connexin 45; Connexin-45; Connexin45; CX 31.9; CX 45; CX31.9; Cx45; CXG1_HUMAN; DKFZp686P0738; Gap junction alpha 7 protein; Gap junction alpha-7 protein; Gap junction gamma 1 protein; Gap junction gamma-1 protein; Gap junction protein alpha 7 45kDa; Gap junction protein alpha 7; Gap junction protein, gamma 1, 45kDa; GJA 7; GJA7; GJC1; GJD3;

免疫原

免疫原:

A synthesized peptide derived from human Connexin 45 / GJA7, corresponding to a region within C-terminal amino acids.

Uniprot:

P36383(CXG1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GJC1(10052)
タンパク質の説明:
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
タンパク質配列:
MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAPLSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDNEEDPMMYPEMELESDKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCSRLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKTSVWI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
89
Bovine
89
Rabbit
89
Sheep
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the connexin family. Gamma-type subfamily.

参考文献

1). Cinnamic acid preserves against myocardial ischemia/reperfusion injury via suppression of NLRP3/Caspase-1/GSDMD signaling pathway. Phytomedicine, 2022 (PubMed: 35320770) [IF=6.7]
2). Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2019 (PubMed: 30483766) [IF=5.7]

Application: WB    Species: rat    Sample: adipose tissue‑derived stem cells

Figure 3.| Expression of related genes by western blot and RT‑qPCR analyses following co‑culture for 5‑7 days. (A) Gene expression levels of HCN4, Cx45 and Cx43 were examined by RT‑qPCR analysis. (B) Protein expression of HCN4, Cx45 and Cx43 was examined using western blotting.
3). Cardioprotective effect of cinnamaldehyde pretreatment on ischemia/ reperfusion injury via inhibiting NLRP3 inflammasome activation and gasdermin D mediated cardiomyocyte pyroptosis. Chemico-Biological Interactions, 2022 (PubMed: 36341777) [IF=4.7]
4). A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells. Molecular Medicine Reports, 2019 (PubMed: 30864738) [IF=3.4]

Application: WB    Species: rat    Sample: ADSCs

Figure 2. |Expression of associated genes by RT‑qPCR and western blotting after co‑culture for 7 days. (A) HCN4, cTnT, Tbx3, Nkx2.5, Cx43 and Cx45 gene expression was examined using RT‑qPCR. (B) HCN4, Cx43 and Cx45 protein expression examined using western blotting.
5). TGF-β1 and connexin-43 expression in neurogenic bladder from rats with sacral spinal cord injury. Neurourology and Urodynamics, 2018 (PubMed: 30070388) [IF=1.8]

Application: WB    Species: rat    Sample: sacral spinal cord

FIGURE 2 |Changes in CX43, pCX43, TGF-β1, Smad3, pSmad3, and CX45 levels examined by Western blot in detrusor after sacral spinal injury. The density of proteins was normalized with the density of the bands of β-actin. CX43 (B) and pCX43 (C) showed a significant decrease but TGF-β1 (D), Smad3 (E), and pSmad3 (F) showed a significant increase in the bladder detrusor after SCI. Those changes were more significant in transection than in hemisection of sacral spine cord. CX45 was not changed among three groups.

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