TAS2R14 Antibody - #DF5159

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製品: TAS2R14 Antibody
カタログ: DF5159
タンパク質の説明: Rabbit polyclonal antibody to TAS2R14
アプリケーション: WB IF/ICC
反応性: Human
分子量: 38 KD; 36kD(Calculated).
ユニプロット: Q9NYV8
RRID: AB_2837508

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
TAS2R14 Antibody detects endogenous levels of total TAS2R14.
RRID:
AB_2837508
引用形式: Affinity Biosciences Cat# DF5159, RRID:AB_2837508.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

T2R14; T2R14_HUMAN; TAS2R14; Taste receptor family B member 1; Taste receptor type 2 member 14; Taste receptor, family B, member 1; Taste receptor, type 2, member 14; TRB1;

免疫原

免疫原:
Uniprot:

Q9NYV8(T2R14_HUMAN) >>Visit HPA database.

遺伝子(ID):
TAS2R14(50840)
発現特異性:
Q9NYV8 T2R14_HUMAN:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in testis (PubMed:16720576).

タンパク質配列:
MGGVIKSIFTFVLIVEFIIGNLGNSFIALVNCIDWVKGRKISSVDRILTALAISRISLVWLIFGSWCVSVFFPALFATEKMFRMLTNIWTVINHFSVWLATGLGTFYFLKIANFSNSIFLYLKWRVKKVVLVLLLVTSVFLFLNIALINIHINASINGYRRNKTCSSDSSNFTRFSSLIVLTSTVFIFIPFTLSLAMFLLLIFSMWKHRKKMQHTVKISGDASTKAHRGVKSVITFFLLYAIFSLSFFISVWTSERLEENLIILSQVMGMAYPSCHSCVLILGNKKLRQASLSVLLWLRYMFKDGEPSGHKEFRESS

PTMs - Q9NYV8 基板として

Site PTM Type Enzyme
T49 Phosphorylation
Y121 Phosphorylation
N153 N-Glycosylation
T164 Phosphorylation
S170 Phosphorylation
T173 Phosphorylation
S223 Phosphorylation
T224 Phosphorylation
Y300 Phosphorylation
S308 Phosphorylation

研究背景

機能:

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 (By similarity).

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in testis.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor T2R family.

研究領域

· Organismal Systems > Sensory system > Taste transduction.

参考文献

1). Biomimetic in vitro respiratory system using smooth muscle cells on ECIS chips for anti-asthma TCMs screening. Analytica Chimica Acta (PubMed: 33926702) [IF=6.2]

Application: IF/ICC    Species: Mouse    Sample: ASM cells

Fig. 2. Identification of TAS2Rs in mouse ASM cells. (A) RT-qPCR of mTAS2Rs expression in mouse ASM cells, with b-actin as internal control. Results were normalized to expression of the Adora1. Data are collected from three independent experiments. (B) Immunostaining with DAPI (blue), anti-TAS2R10, and anti-TAS2R14 receptor antibody (red), scale bar ¼ 20 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
2). Piperine, as a TAS2R14 agonist, stimulates the secretion of glucagon-like peptide-1 in the human enteroendocrine cell line Caco-2. Food & Function (PubMed: 34881772) [IF=6.1]

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