GPR133 Antibody - #DF4947

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製品: GPR133 Antibody
カタログ: DF4947
タンパク質の説明: Rabbit polyclonal antibody to GPR133
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
分子量: 96 KD; 97kD(Calculated).
ユニプロット: Q6QNK2
RRID: AB_2837300

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
GPR133 Antibody detects endogenous levels of total GPR133.
RRID:
AB_2837300
引用形式: Affinity Biosciences Cat# DF4947, RRID:AB_2837300.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DKFZp434B1272; FLJ16770; G protein coupled receptor 133; G protein coupled receptor PGR25; G-protein coupled receptor PGR25; GP133_HUMAN; Gpr133; MGC138512; MGC138514; PGR25; Probable G-protein coupled receptor 133;

免疫原

免疫原:

A synthesized peptide derived from human GPR133, corresponding to a region within the internal amino acids.

Uniprot:

Q6QNK2(AGRD1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADGRD1(283383)
発現特異性:
Q6QNK2 AGRD1_HUMAN:

Up-regulated in CD133(+) cell population of glioblastoma.

タンパク質配列:
MEKLLRLCCWYSWLLLFYYNFQVRGVYSRSQDHPGFQVLASASHYWPLENVDGIHELQDTTGDIVEGKVNKGIYLKEEKGVTLLYYGRYNSSCISKPEQCGPEGVTFSFFWKTQGEQSRPIPSAYGGQVISNGFKVCSSGGRGSVELYTRDNSMTWEASFSPPGPYWTHVLFTWKSKEGLKVYVNGTLSTSDPSGKVSRDYGESNVNLVIGSEQDQAKCYENGAFDEFIIWERALTPDEIAMYFTAAIGKHALLSSTLPSLFMTSTASPVMPTDAYHPIITNLTEERKTFQSPGVILSYLQNVSLSLPSKSLSEQTALNLTKTFLKAVGEILLLPGWIALSEDSAVVLSLIDTIDTVMGHVSSNLHGSTPQVTVEGSSAMAEFSVAKILPKTVNSSHYRFPAHGQSFIQIPHEAFHRHAWSTVVGLLYHSMHYYLNNIWPAHTKIAEAMHHQDCLLFATSHLISLEVSPPPTLSQNLSGSPLITVHLKHRLTRKQHSEATNSSNRVFVYCAFLDFSSGEGVWSNHGCALTRGNLTYSVCRCTHLTNFAILMQVVPLELARGHQVALSSISYVGCSLSVLCLVATLVTFAVLSSVSTIRNQRYHIHANLSFAVLVAQVLLLISFRLEPGTTPCQVMAVLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHRYYYGMGWGFPLLICIISLSFAMDSYGTSNNCWLSLASGAIWAFVAPALFVIVVNIGILIAVTRVISQISADNYKIHGDPSAFKLTAKAVAVLLPILGTSWVFGVLAVNGCAVVFQYMFATLNSLQGLFIFLFHCLLNSEVRAAFKHKTKVWSLTSSSARTSNAKPFHSDLMNGTRPGMASTKLSPWDKSSHSAHRVDLSAV

研究背景

機能:

Orphan receptor. Signals via G(s)-alpha family of G-proteins. Has protumorigenic function especially in glioblastoma.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Up-regulated in CD133(+) cell population of glioblastoma.

タンパク質ファミリー:

A short peptide sequence (termed the Stachel sequence) in the C-terminal part of the extracellular domain (ECD) functions as a tethered agonist. Upon structural changes within the ECD, e.g. due to extracellular ligand binding or mechanical movements, this intramolecular agonist is exposed to the 7 transmembrane region, triggering G-protein activation.

The N-terminal domain and autocatalytic activity of ADGRD1 at the GPCR proteolysis site (GPS) are not required for G-protein coupling activity.

Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.

参考文献

1). Circular RNA circNRIP1 promotes glioma progression by regulating the miR-106a-5p/GPR133 pathway. Scientific reports, 2025 (PubMed: 41225028) [IF=3.8]

Application: WB    Species: human    Sample: U87 and LN229 cell

Fig. 3 The binding relationship between circNRIP1 and miR-106a-5p, as well as GPR133 and miR-106a-5p. (A and B) The predicted binding sites between circNRIP1 and miR-106a-5p, as well as GPR133 3′UTR and miR-106a-5p were exhibited. (C) The interaction between circNRIP1 and miR-106a-5p was confirmed by dual-luciferase reporter assay. (D) The expression of miR-106a-5p in U87 and LN229 cells transfected with si-circNRIP1 or si-NC was detected by qRT-PCR. (E) The correlation between miR-106a-5p and circNRIP1 expression in glioma tissues. (F) The interaction between GPR133 and miR-106a-5p was confirmed by dual-luciferase reporter assay. (G) The mRNA level of GPR133 in U87 and LN229 cells transfected with miR-106a-5p mimic or mimic-NC was detected by qRT-PCR. (H and I) The protein level of GPR133 in U87 and LN229 cells transfected with miR-106a-5p mimic or mimic-NC was detected by western blotting. Data are presented as the mean ± SD of three independent experiments.

Application: IHC    Species: Mouse    Sample:

Fig. 5 Downregulation of circNRIP1 inhibits glioma cell growth in vivo. (A) Image of subcutaneous xenograft tumors (n = 6 for each group). (B) Tumor volume was measured every 2 days. (C) Tumor weight was examined 28 days after injection. (D) Ki67, Fibronectin, N-cadherin, Vimentin, GPR133 IHC staining of xenograft tumors. The scale bars are 100 μm.
2). Targeting GPR133 via miR-106a-5p inhibits the proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) of glioma cells. The International journal of neuroscience, 2023 (PubMed: 37036013) [IF=1.7]

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