EDG3 Antibody - #DF4869

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製品: EDG3 Antibody
カタログ: DF4869
タンパク質の説明: Rabbit polyclonal antibody to EDG3
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
分子量: 40 KD; 42kD(Calculated).
ユニプロット: Q99500
RRID: AB_2837232

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
EDG3 Antibody detects endogenous levels of total EDG3.
RRID:
AB_2837232
引用形式: Affinity Biosciences Cat# DF4869, RRID:AB_2837232.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EDG 3; EDG3; Endothelial differentiation G protein coupled receptor 3; Endothelial differentiation G-protein coupled receptor 3; Endothelial differentiation gene 3; Endothelial differentiation sphingolipid G protein coupled receptor 3; FLJ37523; FLJ93220; G protein coupled receptor endothelial differentiation gene 3; LPB 3; LPB3; MGC71696; OTTHUMP00000215691; S1P receptor 3; S1P receptor Edg 3; S1P receptor Edg-3; S1P receptor EDG3; S1P3; S1pr3; S1PR3_HUMAN; Sphingosine 1 phosphate receptor 3; Sphingosine 1 phosphate receptor Edg 3; Sphingosine 1 phosphate receptor Edg3; Sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3;

免疫原

免疫原:

A synthesized peptide derived from human EDG3, corresponding to a region within N-terminal amino acids.

Uniprot:

Q99500(S1PR3_HUMAN) >>Visit HPA database.

遺伝子(ID):
S1PR3(1903)
発現特異性:
Q99500 S1PR3_HUMAN:

Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.

タンパク質配列:
MATALPPRLQPVRGNETLREHYQYVGKLAGRLKEASEGSTLTTVLFLVICSFIVLENLMVLIAIWKNNKFHNRMYFFIGNLALCDLLAGIAYKVNILMSGKKTFSLSPTVWFLREGSMFVALGASTCSLLAIAIERHLTMIKMRPYDANKRHRVFLLIGMCWLIAFTLGALPILGWNCLHNLPDCSTILPLYSKKYIAFCISIFTAILVTIVILYARIYFLVKSSSRKVANHNNSERSMALLRTVVIVVSVFIACWSPLFILFLIDVACRVQACPILFKAQWFIVLAVLNSAMNPVIYTLASKEMRRAFFRLVCNCLVRGRGARASPIQPALDPSRSKSSSSNNSSHSPKVKEDLPHTAPSSCIMDKNAALQNGIFCN

研究背景

機能:

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

参考文献

1). A2 reactive astrocyte-derived exosomes alleviate cerebral ischemia-reperfusion injury by delivering miR-628. Journal of cellular and molecular medicine, 2024 (PubMed: 39159174) [IF=5.3]

Application: WB    Species: human    Sample: HEK293T cell

FIGURE 3 The expression level of miR-628 and the target genes of miR-628. (A) Expression level of miR-628 varies with time after cerebral I/R (n = 3). (B) Expression level of miR-628 in exosomes of astrocytes with different response types (n = 3). (C) Expression levels of NLRP3, S1PR3 and IRF5 mRNA after intracerebroventricular injection of PBS, miR-628 and NC (n = 3). (D, E) Expression levels of the NLRP3, S1PR3 and IRF5 proteins after intracerebroventricular injection of PBS, miR-628 and NC (n = 3). (F–H) Binding sites of NLRP3, S1PR3, IRF5 and miR-628. (I–K) Luciferase activity of HEK293T cells (n = 3). The results are presented as the means ± SDs. *p 
2). Xiaoyin Jiedu Granules may alleviate psoriasis-like skin diseases in mice by regulating sphingosine 1-phosphate receptor expression and reducing Th17 cells. Heliyon, 2023 (PubMed: 37636348) [IF=4.0]

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