FAM3D Antibody - #DF4832

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製品: FAM3D Antibody
カタログ: DF4832
タンパク質の説明: Rabbit polyclonal antibody to FAM3D
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Rat
予測: Pig, Rabbit
分子量: 24 KD; 25kD(Calculated).
ユニプロット: Q96BQ1
RRID: AB_2837197

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(89%), Rabbit(89%)
クローナリティ:
Polyclonal
特異性:
FAM3D Antibody detects endogenous levels of total FAM3D.
RRID:
AB_2837197
引用形式: Affinity Biosciences Cat# DF4832, RRID:AB_2837197.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cytokine-like protein EF-7; EF7; OIT1; Protein FAM3D precursor; UNQ567/PRO1130;

免疫原

免疫原:

A synthesized peptide derived from human FAM3D, corresponding to a region within C-terminal amino acids.

Uniprot:

Q96BQ1(FAM3D_HUMAN) >>Visit HPA database.

遺伝子(ID):
FAM3D(131177)
発現特異性:
Q96BQ1 FAM3D_HUMAN:

Abundantly expressed in placenta and weakly expressed in small intestine.

タンパク質配列:
MRVSGVLRLLALIFAIVTTWMFIRSYMSFSMKTIRLPRWLAASPTKEIQVKKYKCGLIKPCPANYFAFKICSGAANVVGPTMCFEDRMIMSPVKNNVGRGLNIALVNGTTGAVLGQKAFDMYSGDVMHLVKFLKEIPGGALVLVASYDDPGTKMNDESRKLFSDLGSSYAKQLGFRDSWVFIGAKDLRGKSPFEQFLKNSPDTNKYEGWPELLEMEGCMPPKPF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
89
Pig
89
Horse
78
Bovine
78
Sheep
78
Dog
78
Xenopus
56
Chicken
56
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Abundantly expressed in placenta and weakly expressed in small intestine.

タンパク質ファミリー:

Belongs to the FAM3 family.

参考文献

1). DNA methylation-driven gene FAM3D promotes colorectal cancer growth via the ATF4-SESN2-mTORC1 pathway. Aging, 2024 (PubMed: 39388305) [IF=3.9]

Application: WB    Species: human    Sample:

Figure 1. Methylation, expression and prognosis of FAM3D. (A, B) Correlations between FAM3D mRNA expression and methylation of the FAM3D promoter in the (A) TCGA and (B) GEO databases (GSE106582 + GSE101764). (C, D) Kaplan-Meier curves of (C) OS and (D) DFS based on the methylation of the FAM3D promoter in tumor tissues from the TCGA cohort. (E, F) Relative FAM3D mRNA and protein expression after treatment with a series of concentrations of 5-Aza in (E) CW2 and (F) LS513 cells (differences compared to the DMSO group). (G) The mRNA expression of FAM3D in normal tissues and CRC tissues in the TCGA cohort. (H) The mRNA expression of FAM3D in normal cells and CRC cells in GSE132465. (I) The protein expression of FAM3D in normal tissues and COAD tissues in the CPTAC cohort. (J) Kaplan-Meier curve of OS based on FAM3D mRNA expression in tumor tissues from the TCGA cohort. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.

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