製品: SPON2 Antibody
カタログ: DF4682
タンパク質の説明: Rabbit polyclonal antibody to SPON2
アプリケーション: WB IHC IF/ICC
Cited expt.: IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Chicken, Xenopus
分子量: 37 KD; 36kD(Calculated).
ユニプロット: Q9BUD6
RRID: AB_2837033

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 100ul $280 在庫あり
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Bovine(90%), Horse(90%), Chicken(80%), Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
SPON2 Antibody detects endogenous levels of total SPON2.
RRID:
AB_2837033
引用形式: Affinity Biosciences Cat# DF4682, RRID:AB_2837033.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Differentially expressed in cancerous and non-cancerous lung cells 1; DIL 1; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ22401; FLJ34460; M spondin; Mindin; ORF Names:;antibody;SPON 2; Spon2; SPON2_HUMAN; Spondin 2; Spondin 2 extracellular matrix protein; Spondin-2;

免疫原

免疫原:

A synthesized peptide derived from human SPON2, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q9BUD6 SPON2_HUMAN:

Expressed in normal lung tissue but not in lung carcinoma cell lines.

タンパク質配列:
MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHEVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Xenopus
80
Chicken
80
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in normal lung tissue but not in lung carcinoma cell lines.

参考文献

1). Upregulation of Spondin-2 protein expression correlates with poor prognosis in hepatocellular carcinoma. The Journal of international medical research, 2019 (PubMed: 30318967) [IF=1.4]

Application: IHC    Species: Human    Sample: HCC and liver tissue

Figure 2. Expression of SPON2 in HCC and normal liver tissue. (a) qRT-PCR analysis of SPON2 mRNA expression in 20 paired HCC and matched non-cancerous liver tissues. (b) TMA-IHC analysis of SPON2 protein expression in 107 paired HCC and matched non-cancerous liver tissues.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.