MNT Antibody - #DF4676

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製品: MNT Antibody
カタログ: DF4676
タンパク質の説明: Rabbit polyclonal antibody to MNT
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 62 KD(Observed); 62kD(Calculated).
ユニプロット: Q99583
RRID: AB_2837027

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#DF4676-BP

MNT Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
MNT Antibody detects endogenous levels of total MNT.
RRID:
AB_2837027
引用形式: Affinity Biosciences Cat# DF4676, RRID:AB_2837027.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

bHLHd3; Class D basic helix-loop-helix protein 3; MAD6; Max binding protein MNT; MAX network transcriptional repressor; Max-binding protein MNT; Mnt; MNT_HUMAN; MXD6; Myc antagonist MNT; Protein ROX; ROX;

免疫原

免疫原:

A synthesized peptide derived from human MNT, corresponding to a region within the internal amino acids.

Uniprot:

Q99583(MNT_HUMAN) >>Visit HPA database.

遺伝子(ID):
MNT(4335)
タンパク質配列:
MSIETLLEAARFLEWQAQQQQRAREEQERLRLEQEREQEQKKANSLARLAHTLPVEEPRMEAPPLPLSPPAPPPAPPPPLATPAPLTVIPIPVVTNSPQPLPPPPPLPAAAQPLPLAPRQPALVGAPGLSIKEPAPLPSRPQVPTPAPLLPDSKATIPPNGSPKPLQPLPTPVLTIAPHPGVQPQLAPQQPPPPTLGTLKLAPAEEVKSSEQKKRPGGIGTREVHNKLEKNRRAHLKECFETLKRNIPNVDDKKTSNLSVLRTALRYIQSLKRKEKEYEHEMERLAREKIATQQRLAELKHELSQWMDVLEIDRVLRQTGQPEDDQASTSTASEGEDNIDEDMEEDRAGLGPPKLSHRPQPELLKSTLPPPSTTPAPLPPHPHPHPHSVALPPAHLPVQQQQPQQKTPLPAPPPPPAAPAQTLVPAPAHLVATAGGGSTVIAHTATTHASVIQTVNHVLQGPGGKHIAHIAPSAPSPAVQLAPATPPIGHITVHPATLNHVAHLGSQLPLYPQPVAVSHIAHTLSHQQVNGTAGLGPPATVMAKPAVGAQVVHHPQLVGQTVLNPVTMVTMPSFPVSTLKLA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). MNT inhibits lung adenocarcinoma ferroptosis and chemosensitivity by suppressing SAT1. Communications biology, 2024 (PubMed: 38831092) [IF=5.9]

Application: WB    Species: human    Sample: A549 and PC9 cell

Fig. 1: Identification of MNT involved in LUAD ferroptosis. a, b Volcano plot exhibiting MNT involved in regulating ferroptosis induced by RSL3 and IKE (2.5 μM RSL3 and 8 μM IKE for 5 h). c, d MNT RNA levels in A549 and PC9 cell lines were assessed following treatment with RSL3/IKE (2.5 μM RSL3 and 8 μM IKE for 5 h), DFO + RSL3/IKE, or Ferr-1 + RSL3/IKE. e, f MNT protein levels were evaluated in A549 and PC9 cell lines after treatment with RSL3/IKE (2.5 μM RSL3 and 8 μM IKE for 5 h), DFO + RSL3/IKE, or Ferr-1 + RSL3/IKE. Data were analyzed by Student’s t-test and presented by mean ± SD in triplicate. ns, not significant

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