UBAP2L Antibody - #DF4599

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製品: UBAP2L Antibody
カタログ: DF4599
タンパク質の説明: Rabbit polyclonal antibody to UBAP2L
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 115 KD(Observed); 115kD(Calculated).
ユニプロット: Q14157
RRID: AB_2836963

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#DF4599-BP

UBAP2L Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
UBAP2L Antibody detects endogenous levels of total UBAP2L.
RRID:
AB_2836963
引用形式: Affinity Biosciences Cat# DF4599, RRID:AB_2836963.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ42300; KIAA0144; NICE 4; NICE4; Protein NICE-4; Protein NICE4; Ubap2l; Ubiquitin associated protein 2 like; Ubiquitin-associated protein 2-like; UBP2L_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human UBAP2L, corresponding to a region within the internal amino acids.

Uniprot:

Q14157(UBP2L_HUMAN) >>Visit HPA database.

遺伝子(ID):
UBAP2L(9898)
発現特異性:
Q14157 UBP2L_HUMAN:

Ubiquitous.

タンパク質配列:
MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSGGPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTWNNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQRIDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVSMLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDLKNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPSKSTSAPQMSPGSSDNQSSSPQPAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKNGFSSVQATQLQTTQSVEGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHSSTLSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSLNSGSSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHAYPPQVYGYDDLQMLQTRFPLDYYSIPFPTPTTPLTGRDGSLASNPYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTHHTTQQTFLNPALPPGYSYTSLPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQPSGYGSHGYNTGVSVTSSNTGVPDISGSVYSKTQQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPAPFMHILTPHQQPHSQILHHHLQQDGQTGSGQRSQTSSIPQKPQTNKSAYNSYSWGAN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
73
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs).

PTMs:

Acetylated.

組織特異性:

Ubiquitous.

参考文献

1). Ubiquitin-associated protein 2 like (UBAP2L) enhances growth and metastasis of gastric cancer cells. Bioengineered, 2021 (PubMed: 34823423) [IF=4.2]

Application: WB    Species: Human    Sample: gastric cancer cells

Figure 3. Silencing of UBAP2L repressed proliferation and promoted apoptosis of gastric cancer cells a and b. The mRNA and protein levels of UBAP2L in normal human gastric mucosa cells and gastric cancer cells were determined by real-time PCR and western blot, respectively. c and d. The mRNA and protein levels of UBAP2L in AGS and NCI-N87 cells were detected after transfection of siRNA targeting UBAP2L. e and f. CCK-8 assay was performed for measurement of cell viability in UBAP2L-silenced AGS and NCI-N87 cells. g-i. The cell cycle distribution of AGS and NCI-N87 cells was examined by flow cytometry. j. The proliferation index (PI) of AGS and NCI-N87 cells were calculated based on the cell numbers in each phase of the cell cycle (PI = (cell numbers in G2/M and S phases)/cell numbers in G1/G0 phase). k and l. The apoptosis of AGS and NCI-N87 cells were detected by flow cytometry. (*p < 0.05, **p < 0.01, ***p < 0.001, compared with siRNA NC)

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