製品: SLC28A2 Antibody
カタログ: DF4522
タンパク質の説明: Rabbit polyclonal antibody to SLC28A2
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Rabbit
分子量: 65 KD(Observed); 72kD(Calculated).
ユニプロット: O43868
RRID: AB_2836873

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(92%), Horse(%), Sheep(%), Rabbit(%)
クローナリティ:
Polyclonal
特異性:
SLC28A2 Antibody detects endogenous levels of total SLC28A2.
RRID:
AB_2836873
引用形式: Affinity Biosciences Cat# DF4522, RRID:AB_2836873.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CNT 2; CNT2; Concentrative nucleoside transporter 2; FLJ21468; hCNT2; HsT17153; MGC138252; Na(+)/nucleoside cotransporter 2; S28A2_HUMAN; Slc28a2; Sodium coupled nucleoside transporter 2; Sodium-coupled nucleoside transporter 2; Sodium/nucleoside cotransporter 2; Sodium/purine nucleoside co transporter; Sodium/purine nucleoside co-transporter; Solute carrier family 28 (sodium coupled nucleoside transporter) member 2; Solute carrier family 28 member 2; SPNT; SPNT1;

免疫原

免疫原:

A synthesized peptide derived from human SLC28A2, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
O43868 S28A2_HUMAN:

Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain. Weak expression in lung.

タンパク質配列:
MEKASGRQSIALSTVETGTVNPGLELMEKEVEPEGSKRTDAQGHSLGDGLGPSTYQRRSRWPFSKARSFCKTHASLFKKILLGLLCLAYAAYLLAACILNFQRALALFVITCLVIFVLVHSFLKKLLGKKLTRCLKPFENSRLRLWTKWVFAGVSLVGLILWLALDTAQRPEQLIPFAGICMFILILFACSKHHSAVSWRTVFSGLGLQFVFGILVIRTDLGYTVFQWLGEQVQIFLNYTVAGSSFVFGDTLVKDVFAFQALPIIIFFGCVVSILYYLGLVQWVVQKVAWFLQITMGTTATETLAVAGNIFVGMTEAPLLIRPYLGDMTLSEIHAVMTGGFATISGTVLGAFIAFGVDASSLISASVMAAPCALASSKLAYPEVEESKFKSEEGVKLPRGKERNVLEAASNGAVDAIGLATNVAANLIAFLAVLAFINAALSWLGELVDIQGLTFQVICSYLLRPMVFMMGVEWTDCPMVAEMVGIKFFINEFVAYQQLSQYKNKRLSGMEEWIEGEKQWISVRAEIITTFSLCGFANLSSIGITLGGLTSIVPHRKSDLSKVVVRALFTGACVSLISACMAGILYVPRGAEADCVSFPNTSFTNRTYETYMCCRGLFQSTSLNGTNPPSFSGPWEDKEFSAMALTNCCGFYNNTVCA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Sheep
92
Rabbit
92
Horse
85
Xenopus
54
Chicken
46
Bovine
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Sodium-dependent and purine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain. Weak expression in lung.

タンパク質ファミリー:

Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.

参考文献

1). Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model. NPJ biofilms and microbiomes, 2024 (PubMed: 38509085) [IF=9.2]

2). 5-O-Methylvisammioside, a novel inhibitor of CNT2, improves hyperuricemia by inhibiting intestinal purine nucleoside absorption with a safe profile. Biochemical pharmacology, 2025 (PubMed: 40816627) [IF=5.3]

Application: WB    Species: Mouse    Sample:

Fig. 1. Effects of a high UA environment on CNT2. (A) The cell viability was assayed by stimulating HIEC with different concentrations of UA for 24 h (n = 6). (B-C) Relative mRNA and protein levels of CNT2 were determined by qPCR (n = 6) and WB (n = 3). (D-E) CNT2 expression was detected using confocal microscopy. Red fluorescence intensity represents the amount of CNT2 (scale bar = 50 μm; n = 6). (F) Body weight and sUA of HUA mice modeled for 4 weeks (n = 6). (G,I) IHC staining and positive staining intensity analysis of CNT2. (scale bar = 100 μm; n = 6). (H) H&E staining images of the duodenum, jejunum, and ileum (scale bar = 100 μm; n = 6). *p < 0.05, **p < 0.01, and ***p < 0.001, compared to 0 μM or CON.

3). e5NT inhibitor protects acute restraint stress‐induced depression by regulating nucleoside release in mice. JOURNAL OF PHARMACY AND PHARMACOLOGY, 2020 (PubMed: 32743802) [IF=2.8]

Application: WB    Species: mouse    Sample: hippocampus

Figure 2 |Effects of the expression changes in the expression of nucleoside-metabolizing enzyme induced by ARS in the hippocampus of mice.There were no significant changes in the expression of CNT1, CNT2 (a), ENT1 and ENT2 (b).

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