SH2D5 Antibody - #DF4498

(1)   (3)  
製品: SH2D5 Antibody
カタログ: DF4498
タンパク質の説明: Rabbit polyclonal antibody to SH2D5
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 35 KD(Observed); 47kD(Calculated).
ユニプロット: Q6ZV89
RRID: AB_2836849

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
SH2D5 Antibody detects endogenous levels of total SH2D5.
RRID:
AB_2836849
引用形式: Affinity Biosciences Cat# DF4498, RRID:AB_2836849.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ41242; FLJ41847; FLJ42879; SH2 domain containing 5; SH2 domain-containing protein 5; SH2D5; SH2D5_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human SH2D5, corresponding to a region within C-terminal amino acids.

Uniprot:

Q6ZV89(SH2D5_HUMAN) >>Visit HPA database.

遺伝子(ID):
SH2D5(400745)
タンパク質配列:
MQKAGAGGRRASDCGLAPHRPRCITKFAQYVGSFPVDDLDTQESVWLVQQQLWALKDCPRRRAVILKFSLQGLKIYSGEGEVLLMAHALRRILYSTWCPADCQFAFMARNPRSPASKLFCHLFVGSQPGEVQILHLLLCRSFQLAYLLQHPEERAQPEPCPGPTGEVPLKPLSSSGGLVREPFGRDQLSQNVHALVSFRRLPAEGLVGSGKELPESEGRARHARLGNPYCSPTLVRKKAIRSKVIRSGAYRGCTYETQLQLSAREAFPAAWEAWPRGPGGHSCLVESEGSLTENIWAFAGISRPCALALLRRDVLGAFLLWPELGASGQWCLSVRTQCGVVPHQVFRNHLGRYCLEHLPAEFPSLEALVENHAVTERSLFCPLDMGRLNPTYEEQDCGPPGRPPRTLRPLSHAKSEAELQGLG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May be involved in synaptic plasticity regulation through the control of Rac-GTP levels.

細胞の位置付け:

Cell junction>Synapse>Postsynaptic density.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). SH2D5 promotes lung adenocarcinoma cell metastasis and triggers EMT via activating AKT signaling pathway. PloS one, 2024 (PubMed: 39775732) [IF=3.7]

Application: WB    Species: human    Sample: 16HBE and LUAD cell

Fig 1. High SH2D5 expression in lung adenocarcinoma (LUAD) is associated with poor prognosis. (A) The expression level of SH2D5 in 539 SH2D5 samples and 59 normal samples in The Cancer Genome Atlas (TCGA) database. (B) The expression of SH2D5 in 58 paired TCGA tumors and corresponding adjacent noncancerous tissues. (C, D) Overall survival and disease specific survival in low and high SH2D5 expression patients in TGCA cohort. (E, F) Western blot analysis of SH2D5 expression levels in 16HBE and LUAD cell lines.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.