製品: SHD Antibody
カタログ: DF4496
タンパク質の説明: Rabbit polyclonal antibody to SHD
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Sheep, Dog
分子量: 130 KD(Observed); 38kD(Calculated).
ユニプロット: Q96IW2
RRID: AB_2836847

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(88%), Sheep(88%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
SHD Antibody detects endogenous levels of total SHD.
RRID:
AB_2836847
引用形式: Affinity Biosciences Cat# DF4496, RRID:AB_2836847.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

SH2 domain-containing adapter protein D; SHD; SHD_HUMAN; Src homology 2 domain containing transforming protein D;

免疫原

免疫原:

A synthesized peptide derived from human SHD, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MAKWLRDYLSFGGRRPPPQPPTPDYTESDILRAYRAQKNLDFEDPYEDAESRLEPDPAGPGDSKNPGDAKYGSPKHRLIKVEAADMARAKALLGGPGEELEADTEYLDPFDAQPHPAPPDDGYMEPYDAQWVMSELPGRGVQLYDTPYEEQDPETADGPPSGQKPRQSRMPQEDERPADEYDQPWEWKKDHISRAFAVQFDSPEWERTPGSAKELRRPPPRSPQPAERVDPALPLEKQPWFHGPLNRADAESLLSLCKEGSYLVRLSETNPQDCSLSLRSSQGFLHLKFARTRENQVVLGQHSGPFPSVPELVLHYSSRPLPVQGAEHLALLYPVVTQTP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Sheep
88
Dog
88
Horse
75
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May function as an adapter protein.

PTMs:

Tyrosine phosphorylated by ABL.

参考文献

1). Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis. Chemosphere, 2021 (PubMed: 33297146) [IF=8.1]

Application: WB    Species: Mice    Sample: testis

Fig. 5. IL-17A mediates the effect of excessive F on the testosterone level in serum and the expression of testosterone secretion-related proteins in testis (A) The content of testosterone in serum of mice (n ¼ 13. Three times repetition). (B) The expression of testosterone secretion-related proteins in testis (n ¼ 13. Three times repetition). Statistical analysis was carried out using one-way ANOVA with Dunnett’s test. Error bars denote the mean ± s.e.m. *P < 0.05, **P < 0.01.

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