製品: RNUXA Antibody
カタログ: DF4446
タンパク質の説明: Rabbit polyclonal antibody to RNUXA
アプリケーション: WB IF/ICC
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 48 KD; 44kD(Calculated).
ユニプロット: Q9H814
RRID: AB_2836801

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
RNUXA Antibody detects endogenous levels of total RNUXA.
RRID:
AB_2836801
引用形式: Affinity Biosciences Cat# DF4446, RRID:AB_2836801.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FLJ13193; PHAX; PHAX_HUMAN; Phosphorylated adapter RNA export protein; Phosphorylated adaptor for RNA export; RNA U small nuclear RNA export adapter protein; RNA U, small nuclear RNA export adapter (phosphorylation regulated); RNUXA;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MALEVGDMEDGQLSDSDSDMTVAPSDRPLQLPKVLGGDSAMRAFQNTATACAPVSHYRAVESVDSSEESFSDSDDDSCLWKRKRQKCFNPPPKPEPFQFGQSSQKPPVAGGKKINNIWGAVLQEQNQDAVATELGILGMEGTIDRSRQSETYNYLLAKKLRKESQEHTKDLDKELDEYMHGGKKMGSKEEENGQGHLKRKRPVKDRLGNRPEMNYKGRYEITAEDSQEKVADEISFRLQEPKKDLIARVVRIIGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYIENQKEYENKKAARKRRTQVLGKKMKQAIKSLNFQEDDDTSRETFASDTNEALASLDESQEGHAEAKLEAEEAIEVDHSHDLDIF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
92
Bovine
92
Sheep
92
Chicken
92
Rabbit
92
Xenopus
82
Zebrafish
42
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H814 基板として

Site PTM Type Enzyme
A2 Acetylation
S14 Phosphorylation
S16 Phosphorylation
S18 Phosphorylation
T21 Phosphorylation
S39 Phosphorylation
Y57 Phosphorylation
S65 Phosphorylation
S66 Phosphorylation
S69 Phosphorylation
S71 Phosphorylation
S73 Phosphorylation
S102 Phosphorylation
K105 Acetylation
K112 Acetylation
S146 Phosphorylation
S149 Phosphorylation
T151 Phosphorylation
Y152 Phosphorylation
Y154 Phosphorylation
S164 Phosphorylation
K169 Methylation
Y178 Phosphorylation
K183 Acetylation
K184 Acetylation
S226 Phosphorylation
T296 Phosphorylation
Y316 Phosphorylation
T327 Phosphorylation
K339 Ubiquitination
T349 Phosphorylation
S350 Phosphorylation
T353 Phosphorylation
S356 Phosphorylation
T358 Phosphorylation
S364 Phosphorylation
S368 Phosphorylation
S388 Phosphorylation

研究背景

機能:

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

PTMs:

Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity).

細胞の位置付け:

Nucleus>Nucleoplasm. Nucleus>Cajal body. Cytoplasm.
Note: Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20 and m7G-capped RNA. Interacts with NCBP1/CBP80 (By similarity). Found in a complex with snoRNA. Interacts with NCBP2/CBP20.

タンパク質ファミリー:

Belongs to the PHAX family.

研究領域

· Genetic Information Processing > Translation > RNA transport.

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