製品: ARHGEF7 Antibody
カタログ: DF4436
タンパク質の説明: Rabbit polyclonal antibody to ARHGEF7
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 75-80 KD; 90kD(Calculated).
ユニプロット: Q14155
RRID: AB_2836791

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Dog(100%), Chicken(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
ARHGEF7 Antibody detects endogenous levels of total ARHGEF7.
RRID:
AB_2836791
引用形式: Affinity Biosciences Cat# DF4436, RRID:AB_2836791.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ARHG7; ARHG7_HUMAN; ARHGEF 7; Arhgef7; Beta pix; Beta-Pix; betaPix; betaPix-b; betaPix-c; Cool; COOL-1; COOL1; DKFZp686C12170; DKFZp761K1021; KIAA0142; KIAA0412; mKIAA0142; Nbla10314; P50; P50BP; p85; P85COOL1; P85SPR; PAK interacting exchange factor beta; PAK-interacting exchange factor beta; PAK3; Pak3bp; PIX; PIXB; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor (GEF) 7b; Rho guanine nucleotide exchange factor (GEF7); Rho guanine nucleotide exchange factor 7; SH3 domain containing proline rich protein;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRSESECLSNIREFLRGCGASLRLELLFPPSQPPQHLVTTILLSASTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Pig
91
Bovine
91
Sheep
91
Xenopus
91
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14155 基板として

Site PTM Type Enzyme
S25 Phosphorylation
S123 Phosphorylation
S124 Phosphorylation
T127 Phosphorylation Q5S007 (LRRK2)
S139 Phosphorylation
S153 Phosphorylation
T164 Phosphorylation Q5S007 (LRRK2)
S176 Phosphorylation
K242 Ubiquitination
S244 Phosphorylation
S249 Phosphorylation
K251 Ubiquitination
S252 Phosphorylation
T254 Phosphorylation
S257 Phosphorylation
T264 Phosphorylation
K405 Ubiquitination
K418 Ubiquitination
Y427 Phosphorylation
K442 Ubiquitination
S518 Phosphorylation P05129 (PRKCG) , Q13153 (PAK1)
T528 Phosphorylation
S539 Phosphorylation
K575 Ubiquitination
T580 Phosphorylation
S581 Phosphorylation
S599 Phosphorylation
T603 Phosphorylation
T617 Phosphorylation
Y620 Phosphorylation
S653 Phosphorylation
K675 Acetylation
S676 Phosphorylation
K678 Acetylation
K681 Acetylation
K682 Acetylation
S694 Phosphorylation Q14012 (CAMK1)
S700 Phosphorylation
K702 Ubiquitination
S703 Phosphorylation
T704 Phosphorylation
K715 Ubiquitination
Y720 Phosphorylation
T722 Phosphorylation
S723 Phosphorylation
K725 Ubiquitination
S761 Phosphorylation P05129 (PRKCG)
S763 Phosphorylation
S784 Phosphorylation
Y785 Phosphorylation
S800 Phosphorylation

研究背景

機能:

Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.

PTMs:

Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1 (By similarity). Phosphorylated on Ser-694 by CaMK1; enhancement of GEF activity and downstream activation of RAC1.

細胞の位置付け:

Cell junction>Focal adhesion. Cell projection>Ruffle. Cytoplasm>Cell cortex. Cell projection>Lamellipodium.
Note: Detected at cell adhesions. A small proportion is detected at focal adhesions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with PAK kinases through the SH3 domain. Interacts with GIT1 and TGFB1I1. Interacts with PTK2/FAK1 and RAC1. Interacts with ITCH and PARVB (By similarity). Interacts with unphosphorylated PAK1. Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis. Interacts with FRMPD4 (via N-terminus). Interacts with CaMK1. Interacts with BIN2. Isoform 1 and isoform 5 interact with SNX27. Interacts with YWHAZ (By similarity).

研究領域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.