RGS5 Antibody - #DF4417

Fig. 4: Lipopolysaccharide induces the differentiation of HPCs towards PDGFRα+ CAFs. A, B Pseudotime trajectories of all CAFs revealing 3 branches. The blue, red, green and grey circles indicate cells at the termini of the branches. C Heatmap showing dynamic changes in gene expression in the CAF lineage. The genes were clustered into 3 gene sets, each of which was characterized by specific expression profiles, as depicted by a selection of genes characteristic of each cluster. Rows: GO enrichment analysis of DEGs; columns: cells shown in pseudotime order. D Expression of key regulators plotted along the pseudotime axis. E, F The expression of CAF markers (α-SMA, RGS5, and PDGFRα) was detected in HPCs treated with LPS for 7 days and 14 days by immunofluorescence staining. Nuclei were stained with DAPI (blue). G Fuzzy Cmeans clustering identified 8 distinct temporal patterns of gene expression. The x-axis shows three stages of HPCs (control and treated with LPS for 7 days and 14 days), whereas the y-axis shows the log2-transformed, normalized intensity ratios at each stage. (H Bar graphs showing the pathways identified after KEGG and GO enrichment analysis of genes in Cluster 4 and 5, respectively. I, J Volcano plot showing differentially expressed genes (DEGs) between HPCs treated with LPS for 7 days and untreated HPCs (I, left panel) or between HPCs treated with LPS for 14 days and untreated HPCs. (J left panel). The bar graphs show the Gene Ontology (GO) functional enrichment analysis results for the DEGs between the group treated with LPS for 7 days and the control group and between the group treated with LPS for 14 days and the control group.