RGS12 Antibody | Affinity Biosciences

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RGS12 Antibody - Western blot analysis of lysates from Rat brain, using RGS12 Antibody at 1/1000 dilution.

$Figure 1 - RGS12 is highly expressed in MIRI mice and H/R cells. (A) The MIRI model was developed using a 30 min ischemia and 24 h reperfusion. (B) Echocardiogram, LVEF and LVFS of mice. (C) Quantification of the infarct area in the myocardial tissue and images of infarct tissue. (D) Serum LDH and CK activity. (E) ROS levels (magnification, ×400) and (F) Fe2+ content in the myocardial tissue. RGS12 expression levels in the myocardial tissue was analyzed using (G) reverse-transcription quantitative PCR and western blotting and (H) immunofluorescence. Magnification, ×400. n=6. (I) A cell model of H/R-induced injury was developed using a 12 h serum starvation, 8 h hypoxia and 12 h reoxygenation. RGS12 expression levels in H/R cells was analyzed using (J) reverse transcription quantitative PCR and western blotting and (K) immunofluorescence. Magnification, ×400. n=3. Data were presented as the mean ± SD. MIRI, myocardial ischemia/reperfusion injury; H/R, hypoxia/reoxygenation; RGS12, regulator of G-protein signaling 12; LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LDH, lactate dehydrogenase; CK, creatine kinase; ROS, reactive oxygen species.$

RGS12 Antibody - Figure 1 - RGS12 is highly expressed in MIRI mice and H/R cells.

製品:	RGS12 Antibody
カタログ:	DF4415
タンパク質の説明:	Rabbit polyclonal antibody to RGS12
アプリケーション:	WB IF/ICC
Cited expt.:	WB
反応性:	Human, Mouse, Rat
予測:	Pig, Sheep, Dog
分子量:	156 KD(Observed); 156kD(Calculated).
ユニプロット:	O14924
RRID:	AB_2836770

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#DF4415-BP

RGS12 Peptide

1mg

Antigen/Blocking Peptide MS Report

Antigen/Blocking Peptide HPLC Report

MSDS

説明書

COA

プロトコル

WB Handbook

IHC Protocol

IF/ICC Protocol

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human, Mouse, Rat

予測:

Pig(100%), Sheep(%), Dog(%)

クローナリティ:

Polyclonal

特異性:

RGS12 Antibody detects endogenous levels of total RGS12.

RRID:

AB_2836770
引用形式: Affinity Biosciences Cat# DF4415, RRID:AB_2836770.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

Regulator of G protein signalling 12; RGS12; RGS12_HUMAN; RGS 12;

免疫原

免疫原:

A synthesized peptide derived from human RGS12, corresponding to a region within N-terminal amino acids.

Uniprot:

O14924(RGS12_HUMAN) >>Visit HPA database.

遺伝子(ID):

RGS12(6002)

発現特異性:

O14924 RGS12_HUMAN:

Isoform 3 is brain specific.

タンパク質配列:

O14924 RGS12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFRAGEASKRPLPGPSPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAVNEINVKKASHEDVVKLIGKCSGVLHMVIAEGVGRFESCSSDEEGGLYEGKGWLKPKLDSKALGINRAERVVEEMQSGGIFNMIFENPSLCASNSEPLKLKQRSLSESAATRFDVGHESINNPNPNMLSKEEISKVIHDDSVFSIGLESHDDFALDASILNVAMIVGYLGSIELPSTSSNLESDSLQAIRGCMRRLRAEQKIHSLVTMKIMHDCVQLSTDKAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGSLAQEEEGALRTSCHVFMVDPDLFNHKIHQGIARRFGFECTADPDTNGCLEFPASSLPVLQFISVLYRDMGELIEGMRARAFLDGDADAHQNNSTSSNSDSGIGNFHQEEKSNRVLVVDLGGSSSRHGPGGSAWDGVGGRGAQPWGAPWTGPFCPDPEGSPPFEAAHQTDRFWDLNKHLGPASPVEVPPASLRSSVPPSKRGTVGAGCGFNQRWLPVHVLREWQCGHTSDQDSYTDSTDGWSSINCGTLPPPMSKIPADRYRVEGSFAQPPLNAPKREWSRKAFGMQSIFGPHRNVRKTKEDKKGSKFGRGTGLTQPSQRTSARRSFGRSKRFSITRSLDDLESATVSDGELTGADLKDCVSNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACEYFNHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDVLRAPHPDMFKEQQLQIFNLMKFDSYTRFLKSPLYQECILAEVEGRALPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSWSRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKATKHCCIHLPDGTSCVVAVKAGFSIKDILSGLCERHGINGAAADLFLVGGDKPLVLHQDSSILESRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVARYGLDLSGLLVRLSGEKEPLDLGAPISSLDGQRVVLEEKDPSRGKASADKQKGVPVKQNTAVNSSSRNHSATGEERTLGKSNSIKIKGENGKNARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSTELTLPTPAAVAKGFSKRSATGNGRESASQPGEQWEPVQESSDSPSTSPGSASSPPGPPGTTPPGQKSPSGPFCTPQSPVSLAQEGTAQIWKRQSQEVEAGGIQTVEDEHVAELTLMGEGDISSPNSTLLPPPSTPQEVPGPSRPGSGTHGSRDLPVNRIIDVDLVTGSAPGRDGGIAGAQAGPGRSQASGGPPTSDLPGLGPVPGEPAKPKTSAHHATFV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Horse

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

O14924_RGS12_HUMAN

機能:

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form.

Behaves as a cell cycle-dependent transcriptional repressor, promoting inhibition of S-phase DNA synthesis.

細胞の位置付け:

Nucleus. Cytoplasm. Cell projection>Dendrite. Cell junction>Synapse.

Nucleus matrix.
Note: Also localized to discrete nuclear foci that are distinct from sites of RNA processing, PML nuclear bodies, and PcG domains.

組織特異性:

Isoform 3 is brain specific.

タンパク質ファミリー:

The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.

参考文献

1). RGS12 is a target of penehyclidine hydrochloride that enhances oxidative stress and ferroptosis in a model of myocardial ischemia/reperfusion injury by inhibiting the Nrf2 pathway. International journal of molecular medicine, 2025 (PubMed: 39930821) [IF=5.7]

Application: WB Species: Mouse Sample:

Figure 1 - RGS12 is highly expressed in MIRI mice and H/R cells. (A) The MIRI model was developed using a 30 min ischemia and 24 h reperfusion. (B) Echocardiogram, LVEF and LVFS of mice. (C) Quantification of the infarct area in the myocardial tissue and images of infarct tissue. (D) Serum LDH and CK activity. (E) ROS levels (magnification, ×400) and (F) Fe2+ content in the myocardial tissue. RGS12 expression levels in the myocardial tissue was analyzed using (G) reverse-transcription quantitative PCR and western blotting and (H) immunofluorescence. Magnification, ×400. n=6. (I) A cell model of H/R-induced injury was developed using a 12 h serum starvation, 8 h hypoxia and 12 h reoxygenation. RGS12 expression levels in H/R cells was analyzed using (J) reverse transcription quantitative PCR and western blotting and (K) immunofluorescence. Magnification, ×400. n=3. Data were presented as the mean ± SD. MIRI, myocardial ischemia/reperfusion injury; H/R, hypoxia/reoxygenation; RGS12, regulator of G-protein signaling 12; LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LDH, lactate dehydrogenase; CK, creatine kinase; ROS, reactive oxygen species.

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RGS12 Antibody - #DF4415

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