RGS10 Antibody - #DF4414

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製品: RGS10 Antibody
カタログ: DF4414
タンパク質の説明: Rabbit polyclonal antibody to RGS10
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 20 KD(Observed); 21kD(Calculated).
ユニプロット: O43665
RRID: AB_2836769

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
RGS10 Antibody detects endogenous levels of total RGS10.
RRID:
AB_2836769
引用形式: Affinity Biosciences Cat# DF4414, RRID:AB_2836769.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Regulator of G protein signaling 10; Regulator of G-protein signaling 10; RGS10; RGS10_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human RGS10, corresponding to a region within the internal amino acids.

Uniprot:

O43665(RGS10_HUMAN) >>Visit HPA database.

遺伝子(ID):
RGS10(6001)
タンパク質配列:
MFNRAVSRLSRKRPPSDIHDSDGSSSSSHQSLKSTAKWAASLENLLEDPEGVKRFREFLKKEFSEENVLFWLACEDFKKMQDKTQMQEKAKEIYMTFLSSKASSQVNVEGQSRLNEKILEEPHPLMFQKLQDQIFNLMKYDSYSRFLKSDLFLKHKRTEEEEEDLPDAQTAAKRASRIYNT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the muscarinic acetylcholine receptor CHRM2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Modulates the activity of potassium channels that are activated in response to CHRM2 signaling. Activity on GNAZ is inhibited by palmitoylation of the G-protein.

細胞の位置付け:

Cytoplasm>Cytosol. Nucleus.
Note: Forskolin treatment promotes phosphorylation and translocation to the nucleus.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke. Aging and disease, 2023 (PubMed: 37199734) [IF=7.0]
2). miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10. Experimental and Therapeutic Medicine, 2021 (PubMed: 34737809) [IF=2.4]

Application: WB    Species: Mouse    Sample: podocytes

Figure 3 miR-199b-5p regulates the expression of RGS10. (A) Predicted binding sites of miR-199b-5p on the 3'UTR of RGS10. (B) RGS10 expression level after transient transfection and treatment of podocytes with miR-199b-5p mimic, NC mimic, ADR, ADR+miR-199b-5p mimic or ADR+NC mimic. (C) RGS10 expression level after the transient transfection and treatment of podocytes with miR-199b-5p inhibitor, NC inhibitor, ADR, ADR+miR-199b-5p inhibitor or ADR+NC inhibitor. (D) RGS10 protein expression in podocytes treated as in (B). (E) RGS10 protein expression in podocytes treated as in (C). (F) Expression level of RGS10 was determined by RT-qPCR in ADR-treated podocytes transfected with miR-199b-5p mimic or miR-199b-5p mimic+AdRGS10. (G) Expression of Bax and Bcl-2 was determined by western blotting in ADR-treated podocytes transfected as in (F). Control indicates normal podocytes. n=3 in each group. *P

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