S100A16 Antibody - #DF4353

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製品: S100A16 Antibody
カタログ: DF4353
タンパク質の説明: Rabbit polyclonal antibody to S100A16
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 20 KD; 12kD(Calculated).
ユニプロット: Q96FQ6
RRID: AB_2836721

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MS Report
HPLC Report
MSDS
説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:100-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Bovine(90%), Horse(90%), Sheep(90%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
S100A16 Antibody detects endogenous levels of total S100A16.
RRID:
AB_2836721
引用形式: Affinity Biosciences Cat# DF4353, RRID:AB_2836721.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

2300002L21Rik; AAG13; Aging associated gene 13 protein; Aging associated protein 13; Aging-associated gene 13 protein; AI325039; AI663996; DT1P1A7; MGC17528; Protein S100 A16; Protein S100-A16; Protein S100-F; Protein S100F; S100 calcium binding protein A16; S100 calcium-binding protein A16; S100A16; S100F; S10AG_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human S100A16, corresponding to a region within the internal amino acids.

Uniprot:

Q96FQ6(S10AG_HUMAN) >>Visit HPA database.

遺伝子(ID):
S100A16(140576)
発現特異性:
Q96FQ6 S10AG_HUMAN:

Ubiquitous (PubMed:14684152). Highly expressed in esophagus, adipose tissues and colon. Expressed at lower level in lung, brain, pancreas and skeletal muscle. Expression is up-regulated in tumors of bladder, lung, thyroid gland, pancreas and ovary (PubMed:14684152). Expressed in astrocytes (PubMed:17030513).

タンパク質配列:
MSDCYTELEKAVIVLVENFYKYVSKYSLVKNKISKSSFREMLQKELNHMLSDTGNRKAADKLIQNLDANHDGRISFDEYWTLIGGITGPIAKLIHEQEQQSSS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Chicken
70
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Calcium-binding protein. Binds one calcium ion per monomer. Can promote differentiation of adipocytes (in vitro) (By similarity). Overexpression in preadipocytes increases their proliferation, enhances adipogenesis and reduces insulin-stimulated glucose uptake (By similarity).

細胞の位置付け:

Nucleus>Nucleolus. Cytoplasm.
Note: Primarily nucleolar. A high intracellular calcium level induces nucleolar exit and nucleocytoplasmic transport, whereas a low intracellular calcium level leads to nuclear translocation and accumulation within specific region of nucleoli (PubMed:17030513).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Highly expressed in esophagus, adipose tissues and colon. Expressed at lower level in lung, brain, pancreas and skeletal muscle. Expression is up-regulated in tumors of bladder, lung, thyroid gland, pancreas and ovary. Expressed in astrocytes.

タンパク質ファミリー:

S100A16 proteins, but not other S100 proteins, have only one functional Ca(2+) binding site per monomer. Upon Ca(2+) binding, undergoes conformational changes leading to the exposure of hydrophobic patches which could be implicated in the Ca(2+) -dependent nuclear export. Binds Zn(2+). Ca(2+) and Zn(2+) do not bind to the same site. Does not bind Cu(2+).

Belongs to the S-100 family.

参考文献

1). CAV2 promotes the invasion and metastasis of head and neck squamous cell carcinomas by regulating S100 proteins. Cell death discovery, 2022 (PubMed: 36114176) [IF=7.0]

Application: WB    Species: human    Sample: SCC15 and SCC25 cells

Fig. 3: The S100 protein family affects HNSCC metastasis and is regulated by CAV2. A The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 and SCC25 HNSCC cell lines was evaluated by immunoblotting. B The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 cell lines was evaluated by RT–qPCR. C Alterations in the invasive ability of SCC15 and SCC25 cells following siRNA interference of S100 protein family members. The invasive ability of SCC15 and SCC25 cells was detected using Transwell assays. *, **, *** and **** indicate p 

Application: IHC    Species: human    Sample: SCC15 and SCC25 cells

Fig. 3: The S100 protein family affects HNSCC metastasis and is regulated by CAV2. A The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 and SCC25 HNSCC cell lines was evaluated by immunoblotting. B The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 cell lines was evaluated by RT–qPCR. C Alterations in the invasive ability of SCC15 and SCC25 cells following siRNA interference of S100 protein family members. The invasive ability of SCC15 and SCC25 cells was detected using Transwell assays. *, **, *** and **** indicate p 
2). Activated PRDM1-CREBBP contributes to preeclampsia by regulating apoptosis and invasion of the human trophoblast cells. iScience, 2024 (PubMed: 39759022) [IF=5.8]
3). VDAC1-NF-κB/p65-mediated S100A16 contributes to myocardial ischemia/reperfusion injury by regulating oxidative stress and inflammatory response via calmodulin/CaMKK2/AMPK pathway. European journal of pharmacology, 2025 (PubMed: 39613175) [IF=5.0]

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