製品: NDRG3 Antibody
カタログ: DF4340
タンパク質の説明: Rabbit polyclonal antibody to NDRG3
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 45 KD(Observed); 41kD(Calculated).
ユニプロット: Q9UGV2
RRID: AB_2836708

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(80%), Horse(90%), Sheep(80%), Rabbit(90%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
NDRG3 Antibody detects endogenous levels of total NDRG3.
RRID:
AB_2836708
引用形式: Affinity Biosciences Cat# DF4340, RRID:AB_2836708.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FLJ13556; N myc downstream regulated gene 3; N-myc downstream-regulated gene 3 protein; NDRG family member 3; NDRG3; NDRG3_HUMAN; Protein Ndr3; Protein NDRG3;

免疫原

免疫原:

A synthesized peptide derived from human NDRG3, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q9UGV2 NDRG3_HUMAN:

Ubiquitous. Highly expressed in brain.

タンパク質配列:
MDELQDVQLTEIKPLLNDKNGTRNFQDFDCQEHDIETTHGVVHVTIRGLPKGNRPVILTYHDIGLNHKSCFNAFFNFEDMQEITQHFAVCHVDAPGQQEGAPSFPTGYQYPTMDELAEMLPPVLTHLSLKSIIGIGVGAGAYILSRFALNHPELVEGLVLINVDPCAKGWIDWAASKLSGLTTNVVDIILAHHFGQEELQANLDLIQTYRMHIAQDINQDNLQLFLNSYNGRRDLEIERPILGQNDNKSKTLKCSTLLVVGDNSPAVEAVVECNSRLNPINTTLLKMADCGGLPQVVQPGKLTEAFKYFLQGMGYIPSASMTRLARSRTHSTSSSLGSGESPFSRSVTSNQSDGTQESCESPDVLDRHQTMEVSC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Dog
90
Rabbit
90
Bovine
80
Sheep
80
Xenopus
70
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

組織特異性:

Ubiquitous. Highly expressed in brain.

タンパク質ファミリー:

Belongs to the NDRG family.

参考文献

1). Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017 (PubMed: 28743501) [IF=2.5]

Application: WB    Species: human    Sample:

Fig.2 Protein Expression of NDRD3-Raf-ERK pathway, A2a receptors, Cytochrome C.

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