PITPNB Antibody - #DF4291

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製品: PITPNB Antibody
カタログ: DF4291
タンパク質の説明: Rabbit polyclonal antibody to PITPNB
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 32 KD; 32kD(Calculated).
ユニプロット: P48739
RRID: AB_2836642

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MS Report
HPLC Report
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(82%)
クローナリティ:
Polyclonal
特異性:
PITPNB Antibody detects endogenous levels of total PITPNB.
RRID:
AB_2836642
引用形式: Affinity Biosciences Cat# DF4291, RRID:AB_2836642.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

OTTHUMP00000198846; OTTHUMP00000198847; Phosphatidylinositol transfer protein beta isoform; Phosphatidylinositol transfer protein, beta; PI TP beta; PI-TP-beta; PIPNB_HUMAN; Pitpnb; PtdIns transfer protein beta; PtdInsTP; PtdInsTP beta; VIB1B;

免疫原

免疫原:

A synthesized peptide derived from human PITPNB, corresponding to a region within C-terminal amino acids.

Uniprot:

P48739(PIPNB_HUMAN) >>Visit HPA database.

遺伝子(ID):
PITPNB(23760)
発現特異性:
P48739 PIPNB_HUMAN:

Widely expressed in various tissues including brain.

タンパク質配列:
MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLKNEPYEKDGEKGQYTHKIYHLKSKVPAFVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVHIDIADRSQVEPADYKADEDPALFQSVKTKRGPLGPNWKKELANSPDCPQMCAYKLVTIKFKWWGLQSKVENFIQKQEKRIFTNFHRQLFCWIDKWIDLTMEDIRRMEDETQKELETMRKRGSVRGTSAADV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Chicken
82
Zebrafish
60
Xenopus
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

PTMs:

Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.

細胞の位置付け:

Cytoplasm. Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed in various tissues including brain.

タンパク質ファミリー:

Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.

参考文献

1). Microcolin H, a novel autophagy inducer, exerts potent antitumour activity by targeting PITPα/β. Signal transduction and targeted therapy, 2023 (PubMed: 37963877) [IF=40.8]

Application: WB    Species: human    Sample: HGC-27/MKN-28 cells

Fig. 5 Microcolin H functions by binding PITPα/β. a Western blot analysis of PITPα/β expression in wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2). b Wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2) were treated with various concentrations of microcolin H for 12 h, and cell proliferation was detected by using a CCK-8 assay, n = 3 per group. c Wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2) were treated with microcolin H for 48 h. Then, the cells were cultured for 9 d, and the colonies were counted, n = 3 per group. d Western blot analysis of PITPα/β expressions for wild type (WT) and PITPα/β overexpression HGC-27/MKN-28 cells (OV-PITPα/β). e Wild type (WT) and PITPα/β overexpression HGC-27/MKN-28 cells (OV-PITPα/β) were treated with various concentrations of microcolin H for 12 h, and cell proliferation was detected by using a CCK-8 assay, n = 3 per group. Data are presented as the mean ± SEM. *P 

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