製品: NT Antibody
カタログ: DF4245
タンパク質の説明: Rabbit polyclonal antibody to NT
アプリケーション: WB IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 42 KD(Observed); 38kD(Calculated).
ユニプロット: Q9P121
RRID: AB_2836596

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(100%), Bovine(%), Horse(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
クローナリティ:
Polyclonal
特異性:
NT Antibody detects endogenous levels of total NT.
RRID:
AB_2836596
引用形式: Affinity Biosciences Cat# DF4245, RRID:AB_2836596.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

hNT; IgLON family member 2; IGLON2; Neurotrimin; NT; NTM; NTRI_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human NT, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MGVCGYLFLPWKCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDNRVTRVAWLNRSTILYAGNDKWCLDPRVVLLSNTQTQYSIEIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVSPKIVEISSDISINEGNNISLTCIATGRPEPTVTWRHISPKAVGFVSEDEYLEIQGITREQSGDYECSASNDVAAPVVRRVKVTVNYPPYISEAKGTGVPVGQKGTLQCEASAVPSAEFQWYKDDKRLIEGKKGVKVENRPFLSKLIFFNVSEHDYGNYTCVASNKLGHTNASIMLFGPGAVSEVSNGTSRRAGCVWLLPLLVLHLLLKF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
91
Bovine
91
Dog
91
Chicken
91
Rabbit
91
Xenopus
88
Zebrafish
55
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Neural cell adhesion molecule.

細胞の位置付け:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the immunoglobulin superfamily. IgLON family.

参考文献

1). Novel Potential Markers of Myofibroblast Differentiation Revealed by Single-Cell RNA Sequencing Analysis of Mesenchymal Stromal Cells in Profibrotic and Adipogenic Conditions. Biomedicines, 2023 (PubMed: 36979822) [IF=4.7]

Application: IF/ICC    Species: Human    Sample: adipose tissue

Figure 4. Immunocytochemical evaluation of CHD3 (green), RDH10 (green), NTM (green), and αSMA (red) in the control (Control) MSCs and after incubation in profibrotic (F) or adipogenic conditions (Ad). Fluorescent microscopy, blue staining—nuclei stained with DAPI, scale bar is 50 µm (A). The percentage of CHD3-positive cells, mean ± SE, p value is indicated (B). The percentage of cells with neurotrimin clusters, mean ± SE, p value is indicated (C).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.