NMU Antibody - #DF4238

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製品: NMU Antibody
カタログ: DF4238
タンパク質の説明: Rabbit polyclonal antibody to NMU
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Zebrafish, Bovine, Xenopus
分子量: 22 KD(Observed); 20kD(Calculated).
ユニプロット: P48645
RRID: AB_2836589

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#DF4238-BP

NMU Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse
予測:
Zebrafish(100%), Bovine(%), Xenopus(%)
クローナリティ:
Polyclonal
特異性:
NMU Antibody detects endogenous levels of total NMU.
RRID:
AB_2836589
引用形式: Affinity Biosciences Cat# DF4238, RRID:AB_2836589.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Neuromedin U 25; Neuromedin U; Neuromedin-U-25; NmU 25; NMU; NmU-25; NMU_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human NMU, corresponding to a region within C-terminal amino acids.

Uniprot:

P48645(NMU_HUMAN) >>Visit HPA database.

遺伝子(ID):
NMU(10874)
発現特異性:
P48645 NMU_HUMAN:

Expressed throughout the enteric nervous system with highest levels being found in the jejunum.

タンパク質配列:
MLRTESCRPRSPAGQVAAASPLLLLLLLLAWCAGACRGAPILPQGLQPEQQLQLWNEIDDTCSSFLSIDSQPQASNALEELCFMIMGMLPKPQEQDEKDNTKRFLFHYSKTQKLGKSNVVSSVVHPLLQLVPHLHERRMKRFRVDEEFQSPFASQSRGYFLFRPRNGRRSAGFI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Xenopus
100
Bovine
89
Pig
0
Horse
0
Sheep
0
Dog
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Ligand for receptors NMUR1 and NMUR2 (By similarity). Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder.

Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release although its potency is much lower than that of neuromedin precursor-related peptide 36.

Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release from lactotroph cells in the pituitary gland, probably via the hypothalamic dopaminergic system.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed throughout the enteric nervous system with highest levels being found in the jejunum.

タンパク質ファミリー:

Belongs to the NmU family.

参考文献

1). Expression of NMU, PPBP and GNG4 in colon cancer and their influences on prognosis. Translational Cancer Research, 2022 (PubMed: 36388046) [IF=1.5]

Application: WB    Species: Human    Sample: colon cancer cells

Figure 3 Expression of key genes in colon cancer cells. (A) NMU, GNG4, PPBP and AGT mRNA levels were detected by RT-qPCR. (B) The expression of NMU, GNG4 and PPBP was detected by immunoblotting. *, P
2). NMU Is a Poor Prognostic Biomarker in Patients with Lung Adenocarcinoma. DISEASE MARKERS, 2021 (PubMed: 34336003)

Application: WB    Species: Human    Sample: LUAD cells and lung normal cells

Figure 6 The high NMU expression in LUAD cell lines. (a) qRT-PCR detection of the levels of NMU in LUAD cells and lung normal cells. (b) Western blot analysis to detect the expression of NMU protein in LUAD cells and lung normal cells. (c) The bar chart showing the ratio of the NMU protein to actin by densitometry in the different cells. (∗∗∗p < 0.001 vs. normal 16HBE cell).

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