NPDC1 Antibody - #DF4225

(3)   (6)   Copy Info
製品: NPDC1 Antibody
カタログ: DF4225
タンパク質の説明: Rabbit polyclonal antibody to NPDC1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Dog
分子量: 28 KD(Observed); 35kD(Calculated).
ユニプロット: Q9NQX5
RRID: AB_2836576

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
Control Products

#DF4225-BP

NPDC1 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(90%), Bovine(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
NPDC1 Antibody detects endogenous levels of total NPDC1.
RRID:
AB_2836576
引用形式: Affinity Biosciences Cat# DF4225, RRID:AB_2836576.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AI314472; CAB1; Neural proliferation differentiation and control protein 1; NPDC-1; NPDC1; NPDC1_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human NPDC1, corresponding to a region within the internal amino acids.

Uniprot:

Q9NQX5(NPDC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
NPDC1(56654)
発現特異性:
Q9NQX5 NPDC1_HUMAN:

Strongly expressed in adult brain; especially in hippocampus, frontal lobe and temporal lobe.

タンパク質配列:
MATPLPPPSPRHLRLLRLLLSGLVLGAALRGAAAGHPDVAACPGSLDCALKRRARCPPGAHACGPCLQPFQEDQQGLCVPRMRRPPGGGRPQPRLEDEIDFLAQELARKESGHSTPPLPKDRQRLPEPATLGFSARGQGLELGLPSTPGTPTPTPHTSLGSPVSSDPVHMSPLEPRGGQGDGLALVLILAFCVAGAAALSVASLCWCRLQREIRLTQKADYATAKAPGSPAAPRISPGDQRLAQSAEMYHYQHQRQQMLCLERHKEPPKELDTASSDEENEDGDFTVYECPGLAPTGEMEVRNPLFDHAALSAPLPAPSSPPALP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Dog
90
Bovine
80
Chicken
70
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. Might be involved in transcriptional regulation (By similarity).

細胞の位置付け:

Membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Strongly expressed in adult brain; especially in hippocampus, frontal lobe and temporal lobe.

タンパク質ファミリー:

Belongs to the NPDC1/cab-1 family.

参考文献

1). Partial Replacement of Diet with Dehulled Adlay Ameliorates Hepatic Steatosis, Inflammation, Oxidative Stress, and Gut Dysbiosis in Rats with Nonalcoholic Fatty Liver Disease. Nutrients, 2023 (PubMed: 37892450) [IF=5.9]

Application: WB    Species: Rat    Sample:

Figure 2. Hepatic MDA, glutathione, and oxidative stress-related protein expression of rats at the end of the experiment. (A) Hepatic MDA levels; (B) total GSH levels; (C) GSH/GSSG ratio; and the relative protein expression levels of (D) Nrf2, (E) FPN, (F) NCOA4, and (G) HO-1. Data are presented in terms of the mean ± standard error of mean values; n = 6. One-way analysis of variance was followed by Tukey’s multiple comparison test. Lower-case letters denote significantly different data groups in each panel. C, control diet; N, nonalcoholic fatty liver disease diet; NA, nonalcoholic fatty liver disease diet with dehulled adlay; MDA, malondialdehyde; GSH, glutathione; GSSG, glutathione; Nrf2, nuclear factor erythroid 2-related factor 2; NCOA4, nuclear receptor coactivator 4; FPN, ferroportin; HO-1, heme oxygenase 1. * p < 0.05, compared with Group C; # p < 0.05, compared with Group N.
2). Very low-carbohydrate diet with higher protein ratio improves lipid metabolism and inflammation in rats with diet-induced nonalcoholic fatty liver disease. The Journal of nutritional biochemistry, 2024 (PubMed: 38244701) [IF=4.8]
3). Psoralen alleviates ulcerative colitis by suppressing inflammation, modulating oxidative stress, and regulating ferroptosis. European journal of pharmacology, 2025 (PubMed: 40848885) [IF=4.2]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.