TOMM20 Antibody - #DF4179

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製品: TOMM20 Antibody
カタログ: DF4179
タンパク質の説明: Rabbit polyclonal antibody to TOMM20
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 17 KD(Observed); 16kD(Calculated).
ユニプロット: Q15388
RRID: AB_2836544

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#DF4179-BP

TOMM20 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
TOMM20 Antibody detects endogenous levels of total TOMM20.
RRID:
AB_2836544
引用形式: Affinity Biosciences Cat# DF4179, RRID:AB_2836544.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM20; TOM20_HUMAN; TOMM20; Translocase of outer mitochondrial membrane 20 homolog (yeast); Translocase of outer mitochondrial membrane 20 homolog type II;

免疫原

免疫原:

A synthesized peptide derived from human TOMM20, corresponding to a region within the internal amino acids.

Uniprot:

Q15388(TOM20_HUMAN) >>Visit HPA database.

遺伝子(ID):
TOMM20(9804)
タンパク質配列:
MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
75
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.

PTMs:

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

細胞の位置付け:

Mitochondrion outer membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the Tom20 family.

参考文献

1). MiR-20b-Overexpressed BMSC-Derived Exosomes Target SIRT1/BMP2 to Regulate Mitochondrial Autophagy and Osteogenesis in Osteoporosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2025 (PubMed: 41078321) [IF=4.4]
2). Melatonin Promotes in vitro Development of Vitrified-Warmed Mouse GV Oocytes, Potentially by Modulating Phosphorylation of Drp1. Frontiers in veterinary science, 2021 (PubMed: 34631868) [IF=3.2]

Application: IF/ICC    Species: Mouse    Sample:

Figure 4. Effects of melatonin on mitochondrial mass and mitochondrial function in MII oocytes derived from vitrified-warmed mouse GV oocytes. (A) Representative images of TOMM20 in mouse MII oocytes. Scale bar, 50 μm. (B) The fluorescence intensity of TOMM20 was recorded using ImageJ software. In total, n = 197 MII oocytes were used in this assay (F: n = 53; V: n = 49; VM: n = 48; VMD: n = 47). (C) The expression levels of mitochondrial biogenesis related genes. (D) Representative images of mitochondrial membrane potential (MMP) in mouse MII oocytes. Scale bar, 50 μm. (E) The rate of mitochondrial membrane potential was recorded using ImageJ software. In total, n = 129 MII oocytes were used in this assay (F: n = 32; V: n = 27; VM: n = 36; VMD: n = 34). (F) The ATP content in different groups. In total, n = 120 MII oocytes were used in this assay (F: n = 30; V: n = 30; VM: n = 30; VMD: n = 30). Data are presented as mean ± SEM of three independent experiments. Groups without a common superscript are significantly different (P < 0.05). The four experimental groups: Fresh control (F), vitrification (V), vitrification + MT (VM), and vitrification + Mdivi-1 (VMD).

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