HOXA5 Antibody - #DF4123

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製品: HOXA5 Antibody
カタログ: DF4123
タンパク質の説明: Rabbit polyclonal antibody to HOXA5
アプリケーション: WB IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 30kDa,43kDa(Observed); 29kD(Calculated).
ユニプロット: P20719
RRID: AB_2836488

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#DF4123-BP

HOXA5 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(100%), Bovine(%), Horse(%), Rabbit(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
HOXA5 Antibody detects endogenous levels of total HOXA5.
RRID:
AB_2836488
引用形式: Affinity Biosciences Cat# DF4123, RRID:AB_2836488.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Homeo box 1C; Homeo box A5; Homeobox A5; Homeobox protein Hox-1C; Homeobox protein Hox-A5; Homeobox protein HOXA5; HOX 1; HOX 1.3; HOX 1C; HOX1; HOX1.3; HOX1C; HOXA5; HXA5_HUMAN; MGC9376; OTTHUMP00000217259;

免疫原

免疫原:

A synthesized peptide derived from human HOXA5, corresponding to a region within the internal amino acids.

Uniprot:

P20719(HXA5_HUMAN) >>Visit HPA database.

遺伝子(ID):
HOXA5(3202)
タンパク質配列:
MSSYFVNSFCGRYPNGPDYQLHNYGDHSSVSEQFRDSASMHSGRYGYGYNGMDLSVGRSGSGHFGSGERARSYAASASAAPAEPRYSQPATSTHSPQPDPLPCSAVAPSPGSDSHHGGKNSLSNSSGASADAGSTHISSREGVGTASGAEEDAPASSEQASAQSEPSPAPPAQPQIYPWMRKLHISHDNIGGPEGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSMAAAGGAFRP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
90
Xenopus
75
Chicken
70
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the Antp homeobox family.

参考文献

1). Prenatal PM2.5 exposure impairs spatial learning and memory in male mice offspring: from transcriptional regulation to neuronal morphogenesis. Particle and Fibre Toxicology, 2023 (PubMed: 37081511) [IF=7.2]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5 Transcriptional regulation of neuronal morphological development-associated DEGs in male offspring following prenatal PM2.5 exposure. (a) The numbers of DEGs associated with the morphological development of axons, dendrites, and synapses at different windows, with genes selected based on FDR 
2). HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1. IUBMB LIFE, 2021 (PubMed: 34117711) [IF=3.7]

Application: WB    Species: Mice    Sample: RAW264.7 cells

FIGURE 5 HOXA5 reduced the atherosclerotic lesion formation in carotid arteries. (a, b) Relative mRNA and protein expressions of HOXA5 in carotid arteries with atherosclerosis were determined by QPCR and Western blot when HOXA5 was overexpressed. (c, d) Representative images of carotid arterial sections stained with hematoxylin and eosin (HE) or Oil Red O. Black star: medial layer; black triangle: intimal layer; blue triangle: plaque. & p < .05. n = 6. HOXA5, homeobox A5; QPCR, quantitative real-time reverse transcription PCR

Application: IF/ICC    Species: Mice    Sample: RAW264.7 cells

FIGURE 6 HOXA5 promoted M2 macrophage polarization by the co-regulation of MED1 in carotid arteries. (a) Immunofluorescent images for the colocalization of HOXA5 and CD163. (b, d) Relative mRNA levels of M1 (MARCO and MHCII) and M2 (PPARγ, Arg1, and MRC1) markers were detected by QPCR. (c, e) The levels of IL-6 and IL-10 were determined by ELISA. (f, g) Relative mRNA and protein levels of MED1 were examined using QPCR and Western blot. (h) Immunofluorescent images for the co-localization of MED1 and MAC- 3. & p < .05. n = 6. ELISA, enzyme-linked immunosorbent assay; HOXA5, homeobox A5; MED1, mediator subunit 1; QPCR, quantitative real-time reverse transcription PCR

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