CK1 Antibody - #BF0141

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製品: CK1 Antibody
カタログ: BF0141
タンパク質の説明: Mouse monoclonal antibody to CK1
アプリケーション: WB IHC ELISA
Cited expt.: WB
反応性: Human
分子量: 39kDa; 39kD(Calculated).
ユニプロット: P48729
RRID: AB_2833579

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製品説明

ソース:
Mouse
アプリケーション:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFB1554]
特異性:
CK1 antibody detects endogenous levels of total CK1.
RRID:
AB_2833579
引用形式: Affinity Biosciences Cat# BF0141, RRID:AB_2833579.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer; HLCDGP1; KC1A_HUMAN; PRO2975;

免疫原

免疫原:

Purified recombinant fragment of human CK1 expressed in E. Coli.

Uniprot:

P48729(KC1A_HUMAN) >>Visit HPA database.

遺伝子(ID):
CSNK1A1(1452)
タンパク質の説明:
CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
タンパク質配列:
MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF

研究背景

機能:

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.

細胞の位置付け:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Nucleus speckle. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

参考文献

1). Cannabidiol inhibits invasion and metastasis in colorectal cancer cells by reversing epithelial–mesenchymal transition through the Wnt/β-catenin signaling pathway. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2023 (PubMed: 35960375) [IF=2.7]
2). Cannabidiol inhibits invasion and metastasis in colorectal cancer cells by reversing epithelial-mesenchymal transition through the Wnt/β-catenin signaling pathway. Journal of cancer research and clinical oncology, 2023 (PubMed: 35960375) [IF=2.7]
3). Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury. Oncology Letters, 2018 (PubMed: 29963188) [IF=2.5]

Application: WB    Species: rat    Sample: EnSCs

Figure 2. |Expression of EMA, CK, CD49f, THY‑1, Col I. 5B5 and vimentin in EnSCs. (A) mRNA levels of EMA, CK and CD49f in EnSCs. (B) mRNA levels of THY‑1, Col I, 5B5 and vimentin in EnSCs. (C) Protein expression of EMA, CK and CD49f in EnSCs.

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