製品: Phospho-HBP1 (Ser402) Antibody
カタログ: AF0031
タンパク質の説明: Rabbit polyclonal antibody to Phospho-HBP1 (Ser402)
アプリケーション: WB IF/ICC
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 58kDa; 58kD(Calculated).
ユニプロット: O60381
RRID: AB_2833291

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-HBP1 (Ser402) Antibody detects endogenous levels of HBP1 only when phosphorylated at Sersine 402.
RRID:
AB_2833291
引用形式: Affinity Biosciences Cat# AF0031, RRID:AB_2833291.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FLJ16340; HBP 1; HBP1; HBP1_HUMAN; High mobility group box transcription factor 1; HMG box containing protein 1; HMG box transcription factor 1; HMG box-containing protein 1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
HBP1 trembl
タンパク質配列:
MVWEVKTNQMPNAVQKLLLVMDKRASGMNDSLELLQCNENLPSSPGYNSCDEHMELDDLPELQAVQSDPTQSGMYQLSSDVSHQEYPRSSWNQNTSDIPETTYRENEVDWLTELANIATSPQSPLMQCSFYNRSSPVHIIATSKSLHSYARPPPVSSSSKSEPAFPHHHWKEETPVRHERANSESESGIFCMSSLSDDDDLGWCNSWPSTVWHCFLKGTRLCFHKGSNKEWQDVEDFARAEGCDNEEDLQMGIHKGYGSDGLKLLSHEESVSFGESVLKLTFDPGTVEDGLLTVECKLDHPFYVKNKGWSSFYPSLTVVQHGIPCCEVHIGDVCLPPGHPDAINFDDSGVFDTFKSYDFTPMDSSAVYVLSSMARQRRASLSCGGPGGQDFARSGFSKNCGSPGSSQLSSNSLYAKAVKNHSSGTVSATSPNKCKRPMNAFMLFAKKYRVEYTQMYPGKDNRAISVILGDRWKKMKNEERRMYTLEAKALAEEQKRLNPDCWKRKRTNSGSQQH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60381 基板として

Site PTM Type Enzyme
K16 Ubiquitination
K23 Ubiquitination
S135 Phosphorylation
S156 Phosphorylation
S157 Phosphorylation
S158 Phosphorylation
S159 Phosphorylation
K171 Acetylation
K225 Ubiquitination
K229 Ubiquitination
K255 Ubiquitination
K263 Ubiquitination
K297 Acetylation
K297 Ubiquitination
K305 Acetylation
K305 Ubiquitination
K307 Acetylation
S372 Phosphorylation
S380 Phosphorylation
S382 Phosphorylation
K398 Ubiquitination
S402 Phosphorylation Q15759 (MAPK11) , Q16539 (MAPK14)
K416 Ubiquitination
K419 Acetylation
K419 Ubiquitination
S430 Phosphorylation
K433 Ubiquitination
T453 Phosphorylation
T484 Phosphorylation
K488 Ubiquitination
K495 Ubiquitination
K503 Ubiquitination
S509 Phosphorylation

研究背景

機能:

Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the histone H1.0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.

PTMs:

Ubiquitinated by the CTLH E3 ubiquitin-protein ligase complex, leading to subsequent proteasomal degradation.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Binds the second PAH repeat of SIN3A (Probable). Binds TCF4. Binds RB1.

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