製品: EPHA10 Antibody
カタログ: DF4044
タンパク質の説明: Rabbit polyclonal antibody to EPHA10
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Pig, Bovine, Sheep, Rabbit
分子量: 110 KD(Observed); 110kD(Calculated).
ユニプロット: Q5JZY3
RRID: AB_2836418

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(92%), Bovine(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
EPHA10 Antibody detects endogenous levels of total EPHA10.
RRID:
AB_2836418
引用形式: Affinity Biosciences Cat# DF4044, RRID:AB_2836418.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

EPH receptor A10; EPHA 10; Epha10; EphA10s protein; EPHAA_HUMAN; Ephrin type A receptor 10; Ephrin type-A receptor 10; FLJ16103; FLJ33655; MGC43817;

免疫原

免疫原:

A synthesized peptide derived from human EPHA10, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q5JZY3 EPHAA_HUMAN:

Mainly expressed in testis.

タンパク質配列:
METCAGPHPLRLFLCRMQLCLALLLGPWRPGTAEEVILLDSKASQAELGWTALPSNGWEEISGVDEHDRPIRTYQVCNVLEPNQDNWLQTGWISRGRGQRIFVELQFTLRDCSSIPGAAGTCKETFNVYYLETEADLGRGRPRLGGSRPRKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSRRGFHLAFQDVGACVALVSVRVYYKQCRATVRGLATFPATAAESAFSTLVEVAGTCVAHSEGEPGSPPRMHCGADGEWLVPVGRCSCSAGFQERGDFCEACPPGFYKVSPRRPLCSPCPEHSRALENASTFCVCQDSYARSPTDPPSASCTRPPSAPRDLQYSLSRSPLVLRLRWLPPADSGGRSDVTYSLLCLRCGREGPAGACEPCGPRVAFLPRQAGLRERAATLLHLRPGARYTVRVAALNGVSGPAAAAGTTYAQVTVSTGPGAPWEEDEIRRDRVEPQSVSLSWREPIPAGAPGANDTEYEIRYYEKGQSEQTYSMVKTGAPTVTVTNLKPATRYVFQIRAASPGPSWEAQSFNPSIEVQTLGEAASGSRDQSPAIVVTVVTISALLVLGSVMSVLAIWRRPCSYGKGGGDAHDEEELYFHFKVPTRRTFLDPQSCGDLLQAVHLFAKELDAKSVTLERSLGGGRFGELCCGCLQLPGRQELLVAVHMLRDSASDSQRLGFLAEALTLGQFDHSHIVRLEGVVTRGSTLMIVTEYMSHGALDGFLRRHEGQLVAGQLMGLLPGLASAMKYLSEMGYVHRGLAARHVLVSSDLVCKISGFGRGPRDRSEAVYTTMSGRSPALWAAPETLQFGHFSSASDVWSFGIIMWEVMAFGERPYWDMSGQDVIKAVEDGFRLPPPRNCPNLLHRLMLDCWQKDPGERPRFSQIHSILSKMVQDPEPPKCALTTCPRPPTPLADRAFSTFPSFGSVGAWLEALDLCRYKDSFAAAGYGSLEAVAEMTAQDLVSLGISLAEHREALLSGISALQARVLQLQGQGVQV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Pig
92
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in testis.

タンパク質ファミリー:

The protein kinase domain is predicted to be catalytically inactive.

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

参考文献

1). Comprehensive analysis of Epha10 as a predictor of clinical prognosis and immune checkpoint therapy efficacy in non-small cell lung cancer. Scientific reports, 2024 (PubMed: 39179608) [IF=3.8]

Application: WB    Species: human    Sample:

Figure 8 Validation of the oncogenic role of EphA10 in NSCLC. (A) and (B) Expression of EphA10 in tissue samples by qRT-PCR. (C) Expression of EphA10 in normal lung tissue and NSCLC tissue samples by IHC staining. (D) and (E) The mRNA level of EphA10 was downregulated in EphA10-knockdown cells. (F) The protein level of EphA10 was downregulated in EphA10-knockdown cells. (G) and (H) CCK-8 assays were performed to analyse cell proliferation. (I) and (J) Clonogenic assays were performed to assess cell proliferation. (K) and (L) Transwell assays were performed to analyse cell motility.

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