GNAT1 Antibody - #DF4109

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製品: GNAT1 Antibody
カタログ: DF4109
タンパク質の説明: Rabbit polyclonal antibody to GNAT1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 36 KD; 40kD(Calculated).
ユニプロット: P11488
RRID: AB_2836400

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
GNAT1 Antibody detects endogenous levels of total GNAT1.
RRID:
AB_2836400
引用形式: Affinity Biosciences Cat# DF4109, RRID:AB_2836400.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CSNBAD3; GBT1; GNAT1; GNAT1_HUMAN; GNATR; guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 1; guanine nucleotide binding protein G(T) alpha 1 subunit; Guanine nucleotide-binding protein G(t) subunit alpha-1; Rod specific transducin; rod-type transducin alpha subunit; Transducin alpha-1 chain; transducin, rod-specific;

免疫原

免疫原:
Uniprot:

P11488(GNAT1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GNAT1(2779)
発現特異性:
P11488 GNAT1_HUMAN:

Rod photoreceptor cells (PubMed:1614872). Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium (PubMed:22190596).

タンパク質配列:
MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFFEKIKKAHLSICFPDYDGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
82
Zebrafish
82
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11488 基板として

Site PTM Type Enzyme
G2 Myristoylation
K31 Acetylation
S40 Phosphorylation
K42 Ubiquitination
K47 Ubiquitination
S130 Phosphorylation
Y142 Phosphorylation
S202 Phosphorylation

研究背景

機能:

Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).

細胞の位置付け:

Cell projection>Cilium>Photoreceptor outer segment. Membrane>Peripheral membrane protein. Photoreceptor inner segment.
Note: Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Rod photoreceptor cells. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium.

サブユニット構造:

Heterotrimeric G proteins are composed of 3 subunits alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RHO. Interacts with RGS9 and PDE6G (By similarity). Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons.

タンパク質ファミリー:

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

研究領域

· Organismal Systems > Sensory system > Phototransduction.

参考文献

1). Frequency cumulative effect of subthreshold energy laser-activated remote phosphors irradiation on visual function in guinea pigs. International Journal of Ophthalmology (PubMed: 35186679) [IF=1.4]

Application: IF/ICC    Species: retina    Sample: guinea pigs

Figure 5| High frequency irradiation did not change the density of photoreceptor cells A: Fluorescent staining of biotinylated peanut agglutinin and transducing-alpha in the retina of guinea pigs after high frequency irradiation

Application: IF/ICC    Species: Pig    Sample: photoreceptor cells

Figure 5 High frequency irradiation did not change the density of photoreceptor cells A: Fluorescent staining of biotinylated peanut agglutinin and transducing-alpha in the retina of guinea pigs after high frequency irradiation; B: The density of retinal cone cells of guinea pigs after high frequency irradiation; C: The density of retinal rod cells of guinea pigs after high frequency irradiation. Statistical significance was determined by the paired sample t-test. Data are presented as the mean±SEM. All analyses were performed in duplicate. n=3.

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