NEIL3 Antibody - #DF4042
| 製品: | NEIL3 Antibody |
| カタログ: | DF4042 |
| タンパク質の説明: | Rabbit polyclonal antibody to NEIL3 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 68 KD; 68kD(Calculated). |
| ユニプロット: | Q8TAT5 |
| RRID: | AB_2836395 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF4042, RRID:AB_2836395.
折りたたみ／展開
123; AI449477; BC034753; C85903; DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; EC 3.2.2.-; EC 4.2.99.18; Endonuclease 8-like 3; Endonuclease VIII-like 3; FGP2; FLJ10858; FPG2; hFPG2; hNEI3; MGC27861; MGC36916; Nei endonuclease VIII-like 3 (E. coli); nei endonuclease VIII-like 3; Nei like 3 (E. coli); Nei-like protein 3; NEIL3; NEIL3_HUMAN; OTTHUMP00000218810; RGD1310562;
免疫原
A synthesized peptide derived from human NEIL3, corresponding to a region within C-terminal amino acids.
Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
- Q8TAT5 NEIL3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVEGPGCTLNGEKIRARVLPGQAVTGVRGSALRSLQGRALRLAASTVVVSPQAAALNNDSSQNVLSLFNGYVYSGVETLGKELFMYFGPKALRIHFGMKGFIMINPLEYKYKNGASPVLEVQLTKDLICFFDSSVELRNSMESQQRIRMMKELDVCSPEFSFLRAESEVKKQKGRMLGDVLMDQNVLPGVGNIIKNEALFDSGLHPAVKVCQLTDEQIHHLMKMIRDFSILFYRCRKAGLALSKHYKVYKRPNCGQCHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQPSSKVNISPTISSESKLFSPAHKKPKTAQYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVGKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TAT5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K195 | Sumoylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K244 | Acetylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| K247 | Acetylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| K458 | Sumoylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| T564 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K574 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot |
研究背景
DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
Nucleus.
Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
The N-terminal region (2-281) contains the glycosylase and lyase activities.
Belongs to the FPG family.
研究領域
· Genetic Information Processing > Replication and repair > Base excision repair.
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