DNAJC5 Antibody - #DF4011

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製品: DNAJC5 Antibody
カタログ: DF4011
タンパク質の説明: Rabbit polyclonal antibody to DNAJC5
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 30 KD; 22kD(Calculated).
ユニプロット: Q9H3Z4
RRID: AB_2836371

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(90%)
クローナリティ:
Polyclonal
特異性:
DNAJC5 Antibody detects endogenous levels of total DNAJC5.
RRID:
AB_2836371
引用形式: Affinity Biosciences Cat# DF4011, RRID:AB_2836371.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CLN 4; CLN4; CLN4B; CSP; cysteine string protein alpha; Cysteine string protein; DJC5; DnaJ (Hsp40) homolog subfamily C member 5; DnaJ homolog subfamily C member 5; DNAJC 5; Dnajc5; DNAJC5A; DNJC5_HUMAN; NCL;

免疫原

免疫原:

A synthesized peptide derived from human DNAJC5, corresponding to a region within the internal amino acids.

Uniprot:

Q9H3Z4(DNJC5_HUMAN) >>Visit HPA database.

遺伝子(ID):
DNAJC5(80331)
発現特異性:
Q9H3Z4 DNJC5_HUMAN:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

タンパク質配列:
MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
91
Chicken
90
Rabbit
90
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).

PTMs:

Fatty acylated (By similarity). Heavily palmitoylated in the cysteine string motif (By similarity).

Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58. This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions.

細胞の位置付け:

Membrane>Lipid-anchor. Melanosome. Cell membrane.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

参考文献

1). DNAJC5 promotes cisplatin resistance in epithelial ovarian cancer by autophagy induced by the BiP/IRE1α/XBP1 endoplasmic reticulum stress pathway. Scientific reports, 2025 (PubMed: 41038875) [IF=3.8]

Application: WB    Species: human    Sample: EOC cell

Fig. 2 Overexpression of DNAJC5 promotes DDP resistance in EOC cells in vitro. (a) Expression levels of DNAJC5 in different EOC cell lines. (b) Western blot analysis confirming DNAJC5 protein expression in A2780 cells infected with a lentivirus overexpressing DNAJC5. (c) A2780 and A2780/DNAJC5 cells were incubated with varying concentrations of DDP (50µM, 40µM, 30µM, 20µM, 10µM, 5 µM, 2.5µM, 1.25µM) for 72 h. Cells were then treated with CCK-8 reagent in the dark for 1.5 h, and the final IC50 values were determined. (d,e) Clonogenic assay of A2780/DNAJC5 cells. A total of 300 cells per well were seeded and treated with 3µM DDP after 24 h. After 10 days, colonies were stained with crystal violet and quantified using ImageJ software. The bar graph represents the effect of DDP on the viability of A2780 and A2780/DNAJC5 cells. (f,g) Apoptosis assay of A2780/DNAJC5 cells following treatment with 3µM DDP for 72 h. The bar graph illustrates the average apoptosis rate of A2780/DNAJC5 cells under DDP treatment compared to the Vector control.

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