製品: RPA2 Antibody
カタログ: DF4001
タンパク質の説明: Rabbit polyclonal antibody to RPA2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 128 KD; 128kD(Calculated).
ユニプロット: Q9H9Y6
RRID: AB_2836361

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(82%), Zebrafish(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Chicken(90%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
RPA2 Antibody detects endogenous levels of total RPA2.
RRID:
AB_2836361
引用形式: Affinity Biosciences Cat# DF4001, RRID:AB_2836361.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

A127; DNA directed RNA polymerase I 135kDa polypeptide; DNA directed RNA polymerase I; DNA directed RNA polymerase I subunit RPA2; DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I subunit rpa2; FLJ10816; FLJ21921; MGC131780; OTTHUMP00000161989; OTTHUMP00000203643; OTTHUMP00000203645; POLR 1B; polr1b; Polymerase (RNA) I polypeptide B 128kDa; Polymerase (RNA) I polypeptide B; RNA polymerase I polypeptide B; RNA polymerase I subunit 2; RPA 135; RPA116; RPA135; RPA2; RPA2_HUMAN; Rpo1 2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MDPGSRWRNLPSGPSLKHLTDPSYGIPREQQKAALQELTRAHVESFNYAVHEGLGLAVQAIPPFEFAFKDERISFTILDAVISPPTVPKGTICKEANVYPAECRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVPIMVKSKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQYGVSMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDSFLRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYMLCLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKEKLEGWLVSIKIAFDKKAQKTSVSMNTDNLMRIFTMGIDLTKPFEYLFATGNLRSKTGLGLLQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRRLLPESWGFLCPVHTPDGEPCGLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSECYPVLLDGVMVGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRPVQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCSRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Xenopus
100
Horse
91
Bovine
91
Sheep
91
Dog
91
Chicken
90
Pig
82
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H9Y6 基板として

Site PTM Type Enzyme
K17 Methylation
K17 Ubiquitination
K32 Ubiquitination
K94 Ubiquitination
Y136 Phosphorylation
K144 Ubiquitination
K272 Ubiquitination
K298 Ubiquitination
K406 Ubiquitination
K442 Ubiquitination
K582 Ubiquitination
K693 Methylation
K693 Ubiquitination
K784 Ubiquitination
K786 Ubiquitination
K797 Ubiquitination
K806 Ubiquitination
K844 Ubiquitination
K853 Ubiquitination
K863 Methylation
K863 Ubiquitination
K882 Ubiquitination
Y971 Phosphorylation
Y998 Phosphorylation
Y999 Phosphorylation
K1009 Ubiquitination
S1051 Phosphorylation
K1083 Ubiquitination
K1095 Ubiquitination
Y1118 Phosphorylation

研究背景

機能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

細胞の位置付け:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.

タンパク質ファミリー:

Belongs to the RNA polymerase beta chain family.

研究領域

· Genetic Information Processing > Transcription > RNA polymerase.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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